Utilizing mouse optic nerve crush to examine CNS remyelination.
Antibody
Gene expression
Model Organisms
Molecular biology
Neuroscience
Journal
STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501
Informations de publication
Date de publication:
17 09 2021
17 09 2021
Historique:
entrez:
17
11
2021
pubmed:
18
11
2021
medline:
18
11
2021
Statut:
epublish
Résumé
In developing pro-myelination treatment, an important hurdle is the lack of reliable animal models for assessing de novo myelination in disease settings. We recently showed that regenerated axons in injured optic nerves fail to be myelinated, providing an animal model for this purpose. Here, we describe procedures to promote axonal regeneration, administer optic nerve crush, and assess oligodendrocyte differentiation and maturation into myelination-competent oligodendrocytes. This protocol allows for testing the efficacy of remyelination treatments in an
Identifiants
pubmed: 34786561
doi: 10.1016/j.xpro.2021.100796
pii: S2666-1667(21)00502-5
pmc: PMC8579818
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Pagination
100796Subventions
Organisme : NEI NIH HHS
ID : R01 EY021526
Pays : United States
Organisme : NICHD NIH HHS
ID : P50 HD105351
Pays : United States
Organisme : NEI NIH HHS
ID : F32 EY032779
Pays : United States
Organisme : NINDS NIH HHS
ID : F31 NS098643
Pays : United States
Organisme : NEI NIH HHS
ID : R01 EY026939
Pays : United States
Informations de copyright
© 2021 The Author(s).
Déclaration de conflit d'intérêts
Z.H. is a co-founder and consultant of Myro Therapeutics. Z. H. and J.W. are co-inventors of a patent related to the pro-myelination methods.
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