Fabrication of MSC-laden composites of hyaluronic acid hydrogels reinforced with MEW scaffolds for cartilage repair.
cartilage
hydrogel
interfacial strength
melt-electrowriting
tissue integration
Journal
Biofabrication
ISSN: 1758-5090
Titre abrégé: Biofabrication
Pays: England
ID NLM: 101521964
Informations de publication
Date de publication:
01 12 2021
01 12 2021
Historique:
received:
15
09
2021
accepted:
17
11
2021
pubmed:
18
11
2021
medline:
16
4
2022
entrez:
17
11
2021
Statut:
epublish
Résumé
Hydrogels are of interest in cartilage tissue engineering due to their ability to support the encapsulation and chondrogenesis of mesenchymal stromal cells (MSCs). However, features such as hydrogel crosslink density, which can influence nutrient transport, nascent matrix distribution, and the stability of constructs during and after implantation must be considered in hydrogel design. Here, we first demonstrate that more loosely crosslinked (i.e. softer, ∼2 kPa) norbornene-modified hyaluronic acid (NorHA) hydrogels support enhanced cartilage formation and maturation when compared to more densely crosslinked (i.e. stiffer, ∼6-60 kPa) hydrogels, with a >100-fold increase in compressive modulus after 56 d of culture. While soft NorHA hydrogels mature into neocartilage suitable for the repair of articular cartilage, their initial moduli are too low for handling and they do not exhibit the requisite stability needed to withstand the loading environments of articulating joints. To address this, we reinforced NorHA hydrogels with polycaprolactone (PCL) microfibers produced via melt-electrowriting (MEW). Importantly, composites fabricated with MEW meshes of 400
Identifiants
pubmed: 34788748
doi: 10.1088/1758-5090/ac3acb
pmc: PMC8943711
mid: NIHMS1788945
doi:
Substances chimiques
Hydrogels
0
Hyaluronic Acid
9004-61-9
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIAMS NIH HHS
ID : P30 AR069619
Pays : United States
Organisme : RRD VA
ID : IK6 RX003416
Pays : United States
Organisme : NIAMS NIH HHS
ID : R01 AR077362
Pays : United States
Organisme : NIAMS NIH HHS
ID : T32 AR053461
Pays : United States
Organisme : RRD VA
ID : I01 RX003375
Pays : United States
Informations de copyright
© 2021 IOP Publishing Ltd.
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