Effective methods for increasing coumestrol in soybean sprouts.


Journal

PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081

Informations de publication

Date de publication:
2021
Historique:
received: 12 03 2021
accepted: 03 11 2021
entrez: 18 11 2021
pubmed: 19 11 2021
medline: 22 1 2022
Statut: epublish

Résumé

Coumestrol (CM), a biologically active compound found in Leguminosae plants, provides various human health benefits. To identify easy and effective methods to increase CM content in vegetables, we developed a quantitative analysis method using high-performance liquid chromatography (HPLC). Using this method, we found that soybean sprouts (1.76 ± 0.13 μg/g) have high CM contents among nine vegetables and evaluated the difference in CM contents between two organs of the sprouts: cotyledons and hypocotyls. Next, soybean sprouts were cultivated under different light, temperature, and water conditions and their CM contents were evaluated. CM content was higher in hypocotyls (4.11 ± 0.04 μg/g) than in cotyledons. Cultivating soybean sprouts at 24°C enhanced CM content regardless of light conditions, the growth of fungi and bacteria, and sprout color. Thus, we identified methods of soybean sprout cultivation to increase CM content, which may provide health benefits and enhance value.

Identifiants

pubmed: 34793574
doi: 10.1371/journal.pone.0260147
pii: PONE-D-21-08194
pmc: PMC8601530
doi:

Substances chimiques

Coumestrol V7NW98OB34

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

e0260147

Déclaration de conflit d'intérêts

The authors have declared that no competing interests exist.

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Auteurs

Tomoe Ohta (T)

Faculty of Pharmaceutical Sciences, Department of Pharmacognosy, Nagasaki International University, Sasebo, Nagasaki, Japan.

Takuhiro Uto (T)

Faculty of Pharmaceutical Sciences, Department of Pharmacognosy, Nagasaki International University, Sasebo, Nagasaki, Japan.

Hiromitsu Tanaka (H)

Faculty of Pharmaceutical Sciences, Department of Molecular Biology, Nagasaki International University, Sasebo, Nagasaki, Japan.

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Classifications MeSH