Crystal structures of the elusive Rhizobium etli L-asparaginase reveal a peculiar active site.

Asparaginase / chemistry Bacterial Proteins / chemistry Binding Sites / genetics Biocatalysis Catalytic Domain Cations / chemistry Crystallography, X-Ray Enzyme Stability Hydrogen-Ion Concentration Kinetics Metals / chemistry Models, Molecular Mutation Protein Binding Protein Folding Protein Multimerization Rhizobium etli / enzymology Temperature

Journal

Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555

Informations de publication

Date de publication:
18 11 2021
Historique:
received: 29 06 2021
accepted: 01 11 2021
entrez: 19 11 2021
pubmed: 20 11 2021
medline: 28 12 2021
Statut: epublish

Résumé

Rhizobium etli, a nitrogen-fixing bacterial symbiont of legume plants, encodes an essential L-asparaginase (ReAV) with no sequence homology to known enzymes with this activity. High-resolution crystal structures of ReAV show indeed a structurally distinct, dimeric enzyme, with some resemblance to glutaminases and β-lactamases. However, ReAV has no glutaminase or lactamase activity, and at pH 9 its allosteric asparaginase activity is relatively high, with K

Identifiants

DOI: 10.1038/s41467-021-27105-x PMID: 34795296 PMC: PMC8602277
pubmed: 34795296
doi: 10.1038/s41467-021-27105-x
pii: 10.1038/s41467-021-27105-x
pmc: PMC8602277
doi:

Substances chimiques

Bacterial Proteins 0
Cations 0
Metals 0
Asparaginase EC 3.5.1.1

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

6717

Informations de copyright

© 2021. The Author(s).

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Auteurs

Joanna I Loch (JI)

Department of Crystal Chemistry and Crystal Physics, Faculty of Chemistry, Jagiellonian University, Krakow, Poland.

Barbara Imiolczyk (B)

Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznan, Poland.

Joanna Sliwiak (J)

Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznan, Poland.

Anna Wantuch (A)

Department of Crystal Chemistry and Crystal Physics, Faculty of Chemistry, Jagiellonian University, Krakow, Poland.

Magdalena Bejger (M)

Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznan, Poland.
ORCID: 0000-0003-1660-9745

Miroslaw Gilski (M)

Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznan, Poland.
Department of Crystallography, Faculty of Chemistry, A. Mickiewicz University, Poznan, Poland.
ORCID: 0000-0002-3941-9947

Mariusz Jaskolski (M)

Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznan, Poland. mariuszj@amu.edu.pl.
Department of Crystallography, Faculty of Chemistry, A. Mickiewicz University, Poznan, Poland. mariuszj@amu.edu.pl.
ORCID: 0000-0003-1587-6489

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Classifications MeSH

questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Hydrolases › Amidohydrolases › Asparaginase › Crystal structures of the elusive Rhizobium...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines bactériennes › Crystal structures of the elusive Rhizobium...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Sites de fixation › Crystal structures of the elusive Rhizobium...
questionsmedicales.fr › Métabolisme › Biocatalyse › Crystal structures of the elusive Rhizobium...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Conformation moléculaire › Conformation des protéines › Éléments structuraux des protéines › Domaine catalytique › Crystal structures of the elusive Rhizobium...
questionsmedicales.fr › Produits chimiques inorganiques › Électrolytes › Ions › Cations › Crystal structures of the elusive Rhizobium...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Cristallographie aux rayons X › Crystal structures of the elusive Rhizobium...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Stabilité protéique › Stabilité enzymatique › Crystal structures of the elusive Rhizobium...
questionsmedicales.fr › Phénomènes chimiques › Concentration en ions d'hydrogène › Crystal structures of the elusive Rhizobium...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Cinétique › Crystal structures of the elusive Rhizobium...
questionsmedicales.fr › Produits chimiques inorganiques › Métaux › Crystal structures of the elusive Rhizobium...
questionsmedicales.fr › Techniques d'investigation › Modèles théoriques › Modèles moléculaires › Crystal structures of the elusive Rhizobium...
questionsmedicales.fr › Phénomènes génétiques › Variation génétique › Mutation › Crystal structures of the elusive Rhizobium...
questionsmedicales.fr › Métabolisme › Liaison aux protéines › Crystal structures of the elusive Rhizobium...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Pliage des protéines › Crystal structures of the elusive Rhizobium...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Multimérisation de protéines › Crystal structures of the elusive Rhizobium...
questionsmedicales.fr › Bactéries › Proteobacteria › Alphaproteobacteria › Rhizobiaceae › Rhizobium › Rhizobium etli › Crystal structures of the elusive Rhizobium...
questionsmedicales.fr › Environnement et santé publique › Environnement › Concepts météorologiques › Atmosphère › Temps (météorologie) › Température › Crystal structures of the elusive Rhizobium...