DNA repair protein DNA-PK protects PC12 cells from oxidative stress-induced apoptosis involving AKT phosphorylation.

Animals Apoptosis / physiology Chromones DNA / metabolism DNA Breaks, Double-Stranded / drug effects DNA Repair / drug effects DNA-Activated Protein Kinase / genetics Histones / metabolism Hydrogen Peroxide / metabolism Morpholines Nuclear Proteins / genetics Oxidative Stress / drug effects PC12 Cells Phosphorylation Protein Kinase Inhibitors / pharmacology Proto-Oncogene Proteins c-akt / metabolism Rats
Apoptosis DNA damage DNA repair DNA-PK Oxidative stress

Journal

Molecular biology reports
ISSN: 1573-4978
Titre abrégé: Mol Biol Rep
Pays: Netherlands
ID NLM: 0403234

Informations de publication

Date de publication:
Feb 2022
Historique:
received: 24 03 2021
accepted: 05 11 2021
pubmed: 20 11 2021
medline: 29 3 2022
entrez: 19 11 2021
Statut: ppublish

Résumé

Emerging evidence suggest that DNA-PK complex plays a role in the cellular response to oxidative stress, in addition to its function of double strand break (DSB) repair. In this study we evaluated whether DNA-PK participates in oxidative stress response and whether this role is independent of its function in DNA repair. We used a model of H Taken together, our results show that DNA-PK can protect cells from oxidative stress induced-apoptosis independently from its function of DSB repair enzyme.

Sections du résumé

BACKGROUND BACKGROUND
Emerging evidence suggest that DNA-PK complex plays a role in the cellular response to oxidative stress, in addition to its function of double strand break (DSB) repair. In this study we evaluated whether DNA-PK participates in oxidative stress response and whether this role is independent of its function in DNA repair.
METHODS AND RESULTS RESULTS
We used a model of H
CONCLUSIONS CONCLUSIONS
Taken together, our results show that DNA-PK can protect cells from oxidative stress induced-apoptosis independently from its function of DSB repair enzyme.

Identifiants

DOI: 10.1007/s11033-021-06934-5 PMID: 34797489 PMC: PMC8825611
pubmed: 34797489
doi: 10.1007/s11033-021-06934-5
pii: 10.1007/s11033-021-06934-5
pmc: PMC8825611
doi:

Substances chimiques

2-(morpholin-4-yl)benzo(h)chromen-4-one 0
Chromones 0
Histones 0
Morpholines 0
Nuclear Proteins 0
Protein Kinase Inhibitors 0
DNA 9007-49-2
Hydrogen Peroxide BBX060AN9V
DNA-Activated Protein Kinase EC 2.7.11.1
Prkdc protein, rat EC 2.7.11.1
Proto-Oncogene Proteins c-akt EC 2.7.11.1

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

1089-1101

Subventions

Organisme : Ministero della Salute
ID : Ricerca corrente 2020
Organisme : Ministero dell'Istruzione, dell'Università e della Ricerca
ID : FOE2020

Informations de copyright

© 2021. The Author(s).

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Auteurs

Alessio Cardinale (A)

Molecular and Cellular Neurobiology, IRCCS San Raffaele Roma, Via di Val Cannuta 247, 00166, Rome, Italy.

Serena Saladini (S)

Molecular and Cellular Neurobiology, IRCCS San Raffaele Roma, Via di Val Cannuta 247, 00166, Rome, Italy.

Leonardo Lupacchini (L)

Molecular and Cellular Neurobiology, IRCCS San Raffaele Roma, Via di Val Cannuta 247, 00166, Rome, Italy.

Irene Ruspantini (I)

FAST. Istituto Superiore di Sanita', Viale Regina Elena 299, 00161, Rome, Italy.

Chiara De Dominicis (C)

Molecular and Cellular Neurobiology, IRCCS San Raffaele Roma, Via di Val Cannuta 247, 00166, Rome, Italy.
Department of Neuroscience, Istituto Superiore di Sanita', Viale Regina Elena 299, 00161, Rome, Italy.

Marco Papale (M)

Molecular and Cellular Neurobiology, IRCCS San Raffaele Roma, Via di Val Cannuta 247, 00166, Rome, Italy.

Francesca Silvagno (F)

Department of Oncology, University Torino, via Santena 5 bis, 10126, Torino, Italy.

Enrico Garaci (E)

University San Raffaele, Via di Val Cannuta 247, 00166, Rome, Italy.

Cristiana Mollinari (C)

Department of Neuroscience, Istituto Superiore di Sanita', Viale Regina Elena 299, 00161, Rome, Italy.
Institute of Translational Pharmacology, National Research Council, Via Fosso del Cavaliere 100, 00133, Rome, Italy.

Daniela Merlo (D)

Department of Neuroscience, Istituto Superiore di Sanita', Viale Regina Elena 299, 00161, Rome, Italy. daniela.merlo@iss.it.
ORCID: 0000-0003-4240-5762

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › DNA repair protein DNA-PK protects PC12 cells...
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Mort cellulaire › Mort cellulaire régulée › Apoptose › DNA repair protein DNA-PK protects PC12 cells...
questionsmedicales.fr › Composés hétérocycliques › Composés hétérocycliques à cycles fusionnés › Composés hétérobicycliques › Benzopyranes › 4H-1-Benzopyran-4-ones › DNA repair protein DNA-PK protects PC12 cells...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ADN › DNA repair protein DNA-PK protects PC12 cells...
questionsmedicales.fr › Phénomènes génétiques › Altération de l'ADN › Cassures de l'ADN › Cassures double-brin de l'ADN › DNA repair protein DNA-PK protects PC12 cells...
questionsmedicales.fr › Phénomènes génétiques › Réparation de l'ADN › DNA repair protein DNA-PK protects PC12 cells...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines nucléaires › Protéines liant le poly-adp-ribose › DNA-activated protein kinase › DNA repair protein DNA-PK protects PC12 cells...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Nucléoprotéines › Histone › DNA repair protein DNA-PK protects PC12 cells...
questionsmedicales.fr › Composés chimiques organiques › Radicaux libres › Peroxydes › Peroxyde d'hydrogène › DNA repair protein DNA-PK protects PC12 cells...
questionsmedicales.fr › Composés hétérocycliques › Composés hétéromonocycliques › Oxazines › Morpholines › DNA repair protein DNA-PK protects PC12 cells...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines nucléaires › DNA repair protein DNA-PK protects PC12 cells...
questionsmedicales.fr › Phénomènes physiologiques › Stress physiologique › Stress oxydatif › DNA repair protein DNA-PK protects PC12 cells...
questionsmedicales.fr › Cellules › Cellules chromaffines › Cellules PC12 › DNA repair protein DNA-PK protects PC12 cells...
questionsmedicales.fr › Métabolisme › Phosphorylation › DNA repair protein DNA-PK protects PC12 cells...
questionsmedicales.fr › Actions chimiques et utilisations › Actions pharmacologiques › Mécanismes moléculaires de l'action pharmacologique › Antienzymes › Inhibiteurs de protéines kinases › DNA repair protein DNA-PK protects PC12 cells...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines tumorales › Protéines oncogènes › Protéines proto-oncogènes › Protéines proto-oncogènes c-akt › DNA repair protein DNA-PK protects PC12 cells...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Rats › DNA repair protein DNA-PK protects PC12 cells...