Parp1 promotes sleep, which enhances DNA repair in neurons.
Animals
Female
Male
Animals, Genetically Modified
Behavior, Animal
Brain
/ enzymology
DNA Damage
DNA Repair
DNA-Binding Proteins
/ genetics
Ku Autoantigen
/ genetics
Mice, Inbred C57BL
Neurons
/ enzymology
Poly (ADP-Ribose) Polymerase-1
/ genetics
Rad52 DNA Repair and Recombination Protein
/ genetics
Sleep
Time Factors
Zebrafish
/ embryology
Zebrafish Proteins
/ genetics
DNA damage response
Ku80
NREM
Parp1
Rad52
chromosome dynamics
homeostasis
mice
sleep
zebrafish
Journal
Molecular cell
ISSN: 1097-4164
Titre abrégé: Mol Cell
Pays: United States
ID NLM: 9802571
Informations de publication
Date de publication:
16 12 2021
16 12 2021
Historique:
received:
24
02
2021
revised:
18
10
2021
accepted:
26
10
2021
pubmed:
20
11
2021
medline:
15
2
2022
entrez:
19
11
2021
Statut:
ppublish
Résumé
The characteristics of the sleep drivers and the mechanisms through which sleep relieves the cellular homeostatic pressure are unclear. In flies, zebrafish, mice, and humans, DNA damage levels increase during wakefulness and decrease during sleep. Here, we show that 6 h of consolidated sleep is sufficient to reduce DNA damage in the zebrafish dorsal pallium. Induction of DNA damage by neuronal activity and mutagens triggered sleep and DNA repair. The activity of the DNA damage response (DDR) proteins Rad52 and Ku80 increased during sleep, and chromosome dynamics enhanced Rad52 activity. The activity of the DDR initiator poly(ADP-ribose) polymerase 1 (Parp1) increased following sleep deprivation. In both larva zebrafish and adult mice, Parp1 promoted sleep. Inhibition of Parp1 activity reduced sleep-dependent chromosome dynamics and repair. These results demonstrate that DNA damage is a homeostatic driver for sleep, and Parp1 pathways can sense this cellular pressure and facilitate sleep and repair activity.
Identifiants
pubmed: 34798058
pii: S1097-2765(21)00933-3
doi: 10.1016/j.molcel.2021.10.026
pmc: PMC8688325
mid: NIHMS1754751
pii:
doi:
Substances chimiques
DNA-Binding Proteins
0
Ku Autoantigen
EC 4.2.99.-
Parp1 protein, mouse
EC 2.4.2.30
Poly (ADP-Ribose) Polymerase-1
EC 2.4.2.30
Rad52 DNA Repair and Recombination Protein
0
Rad52 protein, mouse
0
Rad52 protein, zebrafish
0
Xrcc5 protein, mouse
EC 3.6.4.12
Zebrafish Proteins
0
Parp1 protein, zebrafish
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
4979-4993.e7Subventions
Organisme : NIMH NIH HHS
ID : R01 MH116470
Pays : United States
Commentaires et corrections
Type : CommentIn
Type : CommentIn
Informations de copyright
Copyright © 2021 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Interests The authors declare no competing interests.
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