High-resolution mapping of metal ions reveals principles of surface layer assembly in Caulobacter crescentus cells.
Caulobacter crescentus
S-layer
bacteria
cryo-EM
cryo-ET
fluorescence microscopy
long-wavelength X-ray diffraction
metal-ion-binding proteins
Journal
Structure (London, England : 1993)
ISSN: 1878-4186
Titre abrégé: Structure
Pays: United States
ID NLM: 101087697
Informations de publication
Date de publication:
03 02 2022
03 02 2022
Historique:
received:
12
04
2021
revised:
17
08
2021
accepted:
22
10
2021
pubmed:
21
11
2021
medline:
22
3
2022
entrez:
20
11
2021
Statut:
ppublish
Résumé
Surface layers (S-layers) are proteinaceous crystalline coats that constitute the outermost component of most prokaryotic cell envelopes. In this study, we have investigated the role of metal ions in the formation of the Caulobacter crescentus S-layer using high-resolution structural and cell biology techniques, as well as molecular simulations. Utilizing optical microscopy of fluorescently tagged S-layers, we show that calcium ions facilitate S-layer lattice formation and cell-surface binding. We report all-atom molecular dynamics simulations of the S-layer lattice, revealing the importance of bound metal ions. Finally, using electron cryomicroscopy and long-wavelength X-ray diffraction experiments, we mapped the positions of metal ions in the S-layer at near-atomic resolution, supporting our insights from the cellular and simulations data. Our findings contribute to the understanding of how C. crescentus cells form a regularly arranged S-layer on their surface, with implications on fundamental S-layer biology and the synthetic biology of self-assembling biomaterials.
Identifiants
pubmed: 34800371
pii: S0969-2126(21)00380-4
doi: 10.1016/j.str.2021.10.012
pmc: PMC8828063
pii:
doi:
Substances chimiques
Ions
0
Membrane Glycoproteins
0
S-layer proteins
0
Calcium
SY7Q814VUP
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
215-228.e5Subventions
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/M011224/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : MC_U105184326
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 202231/Z/16/Z
Pays : United Kingdom
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/R002517/1
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/P01948X/1
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 208361/Z/17/Z
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/S003339/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/S009213/1
Pays : United Kingdom
Informations de copyright
Copyright © 2021 The Author(s). Published by Elsevier Ltd.. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests The authors declare no competing interests.
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