Towards Real-Time and Affordable Strain-Level Metagenomics-Based Foodborne Outbreak Investigations Using Oxford Nanopore Sequencing Technologies.

Flongle SNP analysis STEC food surveillance metagenomics nanopore outbreak strain-level

Journal

Frontiers in microbiology
ISSN: 1664-302X
Titre abrégé: Front Microbiol
Pays: Switzerland
ID NLM: 101548977

Informations de publication

Date de publication:
2021
Historique:
received: 08 07 2021
accepted: 13 10 2021
entrez: 22 11 2021
pubmed: 23 11 2021
medline: 23 11 2021
Statut: epublish

Résumé

The current routine laboratory practices to investigate food samples in case of foodborne outbreaks still rely on attempts to isolate the pathogen in order to characterize it. We present in this study a proof of concept using Shiga toxin-producing

Identifiants

pubmed: 34803953
doi: 10.3389/fmicb.2021.738284
pmc: PMC8602914
doi:

Types de publication

Journal Article

Langues

eng

Pagination

738284

Informations de copyright

Copyright © 2021 Buytaers, Saltykova, Denayer, Verhaegen, Vanneste, Roosens, Piérard, Marchal and De Keersmaecker.

Déclaration de conflit d'intérêts

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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Auteurs

Florence E Buytaers (FE)

Transversal Activities in Applied Genomics, Sciensano, Brussels, Belgium.
Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium.

Assia Saltykova (A)

Transversal Activities in Applied Genomics, Sciensano, Brussels, Belgium.
Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium.

Sarah Denayer (S)

National Reference Laboratory for Shiga Toxin-Producing Escherichia coli (NRL STEC), Foodborne Pathogens, Sciensano, Brussels, Belgium.

Bavo Verhaegen (B)

National Reference Laboratory for Shiga Toxin-Producing Escherichia coli (NRL STEC), Foodborne Pathogens, Sciensano, Brussels, Belgium.

Kevin Vanneste (K)

Transversal Activities in Applied Genomics, Sciensano, Brussels, Belgium.

Nancy H C Roosens (NHC)

Transversal Activities in Applied Genomics, Sciensano, Brussels, Belgium.

Denis Piérard (D)

National Reference Center for Shiga Toxin-Producing Escherichia coli (NRC STEC), Department of Microbiology and Infection Control, Universitair Ziekenhuis Brussel (UZ Brussel), Vrije Universiteit Brussel (VUB), Brussels, Belgium.

Kathleen Marchal (K)

Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium.
Department of Information Technology, IDlab, IMEC, Ghent University, Ghent, Belgium.

Sigrid C J De Keersmaecker (SCJ)

Transversal Activities in Applied Genomics, Sciensano, Brussels, Belgium.

Classifications MeSH