Identification of ClpP Dual Isoform Disruption as an Antisporulation Strategy for Clostridioides difficile.
Bacterial Proteins
/ antagonists & inhibitors
Bortezomib
/ pharmacology
Clostridioides difficile
/ chemistry
Clostridium Infections
/ microbiology
Gene Editing
/ methods
Gene Expression Regulation, Bacterial
Humans
Mutation
Phenotype
Protein Isoforms
/ genetics
Spores, Bacterial
/ genetics
Virulence
C. difficile
CRISPR-Cas
anaerobic microbiology
bortezomib
caseinolytic protease P
sporulation
Journal
Journal of bacteriology
ISSN: 1098-5530
Titre abrégé: J Bacteriol
Pays: United States
ID NLM: 2985120R
Informations de publication
Date de publication:
15 02 2022
15 02 2022
Historique:
pubmed:
23
11
2021
medline:
23
2
2022
entrez:
22
11
2021
Statut:
ppublish
Résumé
The Gram-positive bacterium Clostridioides difficile is a primary cause of hospital-acquired diarrhea, threatening both immunocompromised and healthy individuals. An important aspect of defining mechanisms that drive C. difficile persistence and virulence relies on developing a more complete understanding of sporulation. C. difficile sporulation is the single determinant of transmission and complicates treatment and prevention due to the chemical and physical resilience of spores. By extension, the identification of druggable targets that significantly attenuate sporulation would have a significant impact on thwarting C. difficile infection. By use of a new CRISPR-Cas9 nickase genome editing methodology, stop codons were inserted early in the coding sequence for
Identifiants
pubmed: 34807726
doi: 10.1128/JB.00411-21
pmc: PMC8846446
doi:
Substances chimiques
Bacterial Proteins
0
Protein Isoforms
0
Bortezomib
69G8BD63PP
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0041121Subventions
Organisme : NIGMS NIH HHS
ID : P20 GM103640
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI119048
Pays : United States
Organisme : NIAID NIH HHS
ID : R01AI119048
Pays : United States
Organisme : NIGMS NIH HHS
ID : P20GM103640
Pays : United States
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