High-resolution epitope mapping and characterization of SARS-CoV-2 antibodies in large cohorts of subjects with COVID-19.
Journal
Communications biology
ISSN: 2399-3642
Titre abrégé: Commun Biol
Pays: England
ID NLM: 101719179
Informations de publication
Date de publication:
22 11 2021
22 11 2021
Historique:
received:
25
02
2021
accepted:
22
10
2021
entrez:
23
11
2021
pubmed:
24
11
2021
medline:
15
12
2021
Statut:
epublish
Résumé
As Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) continues to spread, characterization of its antibody epitopes, emerging strains, related coronaviruses, and even the human proteome in naturally infected patients can guide the development of effective vaccines and therapies. Since traditional epitope identification tools are dependent upon pre-defined peptide sequences, they are not readily adaptable to diverse viral proteomes. The Serum Epitope Repertoire Analysis (SERA) platform leverages a high diversity random bacterial display library to identify proteome-independent epitope binding specificities which are then analyzed in the context of organisms of interest. When evaluating immune response in the context of SARS-CoV-2, we identify dominant epitope regions and motifs which demonstrate potential to classify mild from severe disease and relate to neutralization activity. We highlight SARS-CoV-2 epitopes that are cross-reactive with other coronaviruses and demonstrate decreased epitope signal for mutant SARS-CoV-2 strains. Collectively, the evolution of SARS-CoV-2 mutants towards reduced antibody response highlight the importance of data-driven development of the vaccines and therapies to treat COVID-19.
Identifiants
pubmed: 34811480
doi: 10.1038/s42003-021-02835-2
pii: 10.1038/s42003-021-02835-2
pmc: PMC8608966
doi:
Substances chimiques
Antibodies, Viral
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1317Subventions
Organisme : NCATS NIH HHS
ID : UL1 TR001863
Pays : United States
Investigateurs
Kelly Anastasio
(K)
Michael H Askenase
(MH)
Natasha C Balkcom
(NC)
Maria Batsu
(M)
Santos Bermejo
(S)
Kristina Brower
(K)
Molly L Bucklin
(ML)
Staci Cahill
(S)
Yiyun Cao
(Y)
Michael Chiorazzi
(M)
Caitlin J Chun
(CJ)
Rupak Datta
(R)
Giuseppe DeIuliis
(G)
Coriann E Dorgay
(CE)
Rebecca Earnest
(R)
John Fournier
(J)
Bertie Geng
(B)
Ryan Handoko
(R)
William Khoury-Hanold
(W)
Roy Herbst
(R)
Lynda Knaggs
(L)
Maxine Kuang
(M)
Sarah Lapidus
(S)
Zitong Lin
(Z)
Peiwen Lu
(P)
Tianyang Mao
(T)
Anjelica Martin
(A)
Irene Matos
(I)
David McDonald
(D)
Maksym Minasyan
(M)
Adam J Moore
(AJ)
Nida Naushad
(N)
Allison Nelson
(A)
Jessica Nouws
(J)
Angela Nunez
(A)
Hong-Jai Park
(HJ)
Xiaohua Peng
(X)
Alexander James Robertson
(AJ)
Tyler Rice
(T)
Kadi-Ann Rose
(KA)
Wade Schulz
(W)
Lorenzo Sewanan
(L)
Lokesh Sharma
(L)
Denise Shepard
(D)
Julio Silva
(J)
Michael Simonov
(M)
Mikhail Smolgovsky
(M)
Nicole Sonnert
(N)
Ariktha Srivathsan
(A)
Yvette Strong
(Y)
Codruta Todeasa
(C)
Jordan Valdez
(J)
Sofia Velazquez
(S)
Pavithra Vijayakumar
(P)
Elizabeth B White
(EB)
Alice Zhao
(A)
Informations de copyright
© 2021. The Author(s).
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