Recombinant Escherichia coli Cell Lysates as a Low-Cost Alternative for Vaccines Against Veterinary Clostridial Diseases.
Cell lysate supernatant
Clostridiosis
Inclusion bodies
Recombinant antigens
Recombinant vaccines
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2022
2022
Historique:
entrez:
24
11
2021
pubmed:
25
11
2021
medline:
21
1
2022
Statut:
ppublish
Résumé
This chapter describes a practical, industry-friendly, and efficient vaccine protocol based on the use of Escherichia coli cell fractions (inclusion bodies or cell lysate supernatant) containing the recombinant antigen. This approach was characterized and evaluated in laboratory and farm animals by the seroneutralization assay in mice, thereby showing to be an excellent alternative to induce a protective immune response against clostridial diseases.
Identifiants
pubmed: 34816401
doi: 10.1007/978-1-0716-1888-2_6
doi:
Substances chimiques
Bacterial Vaccines
0
Escherichia coli Vaccines
0
Vaccines, Synthetic
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
105-115Informations de copyright
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
Références
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