Bi-Reporter Vaccinia Virus for Tracking Viral Infections
GFP
NanoLuc
Scarlet
bioluminescence
fluorescence
in vitro
in vivo
in vivo imaging
luciferase
reporter genes
vaccinia virus
Journal
Microbiology spectrum
ISSN: 2165-0497
Titre abrégé: Microbiol Spectr
Pays: United States
ID NLM: 101634614
Informations de publication
Date de publication:
22 12 2021
22 12 2021
Historique:
pubmed:
25
11
2021
medline:
22
2
2022
entrez:
24
11
2021
Statut:
ppublish
Résumé
Recombinant viruses expressing reporter genes allow visualization and quantification of viral infections and can be used as valid surrogates to identify the presence of the virus in infected cells and animal models. However, one of the limitations of recombinant viruses expressing reporter genes is the use of either fluorescent or luciferase proteins that are used alternatively for different purposes. Vaccinia virus (VV) is widely used as a viral vector, including recombinant (r)VV singly expressing either fluorescent or luciferase reporter genes that are useful for specific purposes. In this report, we engineered two novel rVV stably expressing both fluorescent (Scarlet or GFP) and luciferase (Nluc) reporter genes from different loci in the viral genome.
Identifiants
pubmed: 34817228
doi: 10.1128/Spectrum.01601-21
pmc: PMC8612144
doi:
Substances chimiques
Vaccines, Synthetic
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0160121Références
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