Site-Specific Labeling of Endogenous Proteins Using CoLDR Chemistry.


Journal

Journal of the American Chemical Society
ISSN: 1520-5126
Titre abrégé: J Am Chem Soc
Pays: United States
ID NLM: 7503056

Informations de publication

Date de publication:
08 12 2021
Historique:
pubmed: 25 11 2021
medline: 16 12 2021
entrez: 24 11 2021
Statut: ppublish

Résumé

Chemical modifications of native proteins can affect their stability, activity, interactions, localization, and more. However, there are few nongenetic methods for the installation of chemical modifications at a specific protein site in cells. Here we report a covalent ligand directed release (CoLDR) site-specific labeling strategy, which enables the installation of a variety of functional tags on a target protein while releasing the directing ligand. Using this approach, we were able to label various proteins such as BTK, K-Ras

Identifiants

pubmed: 34817989
doi: 10.1021/jacs.1c06167
pmc: PMC8662641
doi:

Substances chimiques

Fluorescent Dyes 0
KRAS protein, human 0
Ligands 0
Piperidines 0
Pyrimidines 0
ibrutinib 1X70OSD4VX
Agammaglobulinaemia Tyrosine Kinase EC 2.7.10.2
Coronavirus Papain-Like Proteases EC 3.4.22.2
papain-like protease, SARS-CoV-2 EC 3.4.22.2
Proto-Oncogene Proteins p21(ras) EC 3.6.5.2
Adenine JAC85A2161
evobrutinib ZA45457L1K

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

20095-20108

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Auteurs

Rambabu N Reddi (RN)

Department of Chemical and Structural Biology, The Weizmann Institute of Science, Rehovot, 7610001, Israel.

Adi Rogel (A)

Department of Chemical and Structural Biology, The Weizmann Institute of Science, Rehovot, 7610001, Israel.

Efrat Resnick (E)

Department of Chemical and Structural Biology, The Weizmann Institute of Science, Rehovot, 7610001, Israel.

Ronen Gabizon (R)

Department of Chemical and Structural Biology, The Weizmann Institute of Science, Rehovot, 7610001, Israel.

Pragati Kishore Prasad (PK)

Department of Chemical and Structural Biology, The Weizmann Institute of Science, Rehovot, 7610001, Israel.

Neta Gurwicz (N)

Department of Immunology, The Weizmann Institute of Science, Rehovot, 7610001, Israel.

Haim Barr (H)

Wohl Institute for Drug Discovery of the Nancy and Stephen Grand Israel National Center for Personalized Medicine, The Weizmann Institute of Science, Rehovot, 7610001, Israel.

Ziv Shulman (Z)

Department of Immunology, The Weizmann Institute of Science, Rehovot, 7610001, Israel.

Nir London (N)

Department of Chemical and Structural Biology, The Weizmann Institute of Science, Rehovot, 7610001, Israel.

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Classifications MeSH