Fusion-Induced Growth of Biomimetic Polymersomes: Behavior of Poly(dimethylsiloxane)-Poly(ethylene oxide) Vesicles in Saline Solutions Under High Agitation.
biomimetics
fusion
microfluidics
polymersomes
vesicles
Journal
Macromolecular rapid communications
ISSN: 1521-3927
Titre abrégé: Macromol Rapid Commun
Pays: Germany
ID NLM: 9888239
Informations de publication
Date de publication:
Mar 2022
Mar 2022
Historique:
received:
03
11
2021
pubmed:
26
11
2021
medline:
9
3
2022
entrez:
25
11
2021
Statut:
ppublish
Résumé
Giant unilamellar vesicles serve as membrane models and primitive mockups of natural cells. With respect to the latter use, amphiphilic polymers can be used to replace phospholipids in order to introduce certain favorable properties, ultimately allowing for the creation of truly synthetic cells. These new properties also enable the employment of new preparation procedures that are incompatible with the natural amphiphiles. Whereas the growth of lipid compartments to micrometer dimensions has been well established, growth of their synthetic analogs remains underexplored. Here, the influence of experimental parameters like salt type/concentration and magnitude of agitation on the fusion of nanometer-sized vesicles made of poly(dimethylsiloxane)-poly(ethylene oxide) graft copolymer (PDMS-g-PEO) is investigated in detail. To this end, dynamic light scattering, microscopy, and membrane mixing assays are employed, and the process at different time and length scales is analyzed. This optimized method is used as an easy tool to obtain giant vesicles, equipped with membrane and cytosolic biomachinery, in the presence of salts at physiological concentrations.
Identifiants
pubmed: 34820929
doi: 10.1002/marc.202100712
doi:
Substances chimiques
Dimethylpolysiloxanes
0
Polymers
0
Polyethylene Glycols
3WJQ0SDW1A
baysilon
63148-62-9
Ethylene Oxide
JJH7GNN18P
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2100712Subventions
Organisme : Max-Planck-Gesellschaft
Organisme : Bundesministerium für Bildung und Forschung
Informations de copyright
© 2021 The Authors. Macromolecular Rapid Communications published by Wiley-VCH GmbH.
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