A mathematical model of hiPSC cardiomyocytes electromechanics.
3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester
/ pharmacology
Action Potentials
/ drug effects
Calcium Channel Agonists
/ pharmacology
Calcium Channel Blockers
/ pharmacology
Computer Simulation
Electrophysiological Phenomena
/ drug effects
Humans
Induced Pluripotent Stem Cells
/ physiology
Models, Theoretical
Myocardial Contraction
/ drug effects
Myocytes, Cardiac
/ drug effects
Verapamil
/ pharmacology
action potential
contractility
drug test
human stem cell-derived cardiomyocyte
immature cardiomyocytes
in silico modeling
Journal
Physiological reports
ISSN: 2051-817X
Titre abrégé: Physiol Rep
Pays: United States
ID NLM: 101607800
Informations de publication
Date de publication:
11 2021
11 2021
Historique:
revised:
18
10
2021
received:
05
08
2021
accepted:
02
11
2021
entrez:
26
11
2021
pubmed:
27
11
2021
medline:
17
3
2022
Statut:
ppublish
Résumé
Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) are becoming instrumental in cardiac research, human-based cell level cardiotoxicity tests, and developing patient-specific care. As one of the principal functional readouts is contractility, we propose a novel electromechanical hiPSC-CM computational model named the hiPSC-CM-CE. This model comprises a reparametrized version of contractile element (CE) by Rice et al., 2008, with a new passive force formulation, integrated into a hiPSC-CM electrophysiology formalism by Paci et al. in 2020. Our simulated results were validated against in vitro data reported for hiPSC-CMs at matching conditions from different labs. Specifically, key action potential (AP) and calcium transient (CaT) biomarkers simulated by the hiPSC-CM-CE model were within the experimental ranges. On the mechanical side, simulated cell shortening, contraction-relaxation kinetic indices (RT
Identifiants
pubmed: 34825519
doi: 10.14814/phy2.15124
pmc: PMC8617339
doi:
Substances chimiques
Calcium Channel Agonists
0
Calcium Channel Blockers
0
3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester
71145-03-4
Verapamil
CJ0O37KU29
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e15124Informations de copyright
© 2021 The Authors. Physiological Reports published by Wiley Periodicals LLC on behalf of The Physiological Society and the American Physiological Society.
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