Simultaneous Determination of Vitamins D3 (Calcitriol, Cholecalciferol) and K2 (Menaquinone-4 and Menaquinone-7) in Dietary Supplements by UHPLC.
UHPLC
calcitriol
cholecalciferol
menaquinone-4
menaquinone-7
method validation
Journal
Molecules (Basel, Switzerland)
ISSN: 1420-3049
Titre abrégé: Molecules
Pays: Switzerland
ID NLM: 100964009
Informations de publication
Date de publication:
19 Nov 2021
19 Nov 2021
Historique:
received:
29
10
2021
revised:
13
11
2021
accepted:
17
11
2021
entrez:
27
11
2021
pubmed:
28
11
2021
medline:
15
12
2021
Statut:
epublish
Résumé
The content and composition of dietary supplements is of great interest due to their increasing consumption and variety of available brand offered in the market. Accurate determination of vitamins is important for the improvement of dietary supplement quality and nutrition assessments. In this regard, the simultaneous determination of vitamin D3 (calcitriol-CT and cholecalciferol-CHL) and K2 (menaquinone-4-MK-4 and menaquinone-7-MK-7) in dietary supplements was developed by using ultra-high-pressure liquid chromatography (UHPLC). The overall runtime per sample was above 35 min, with the retention times of 2.40, 6.59, 7.06, and 32.6 min for vitamin D3 (CT and CHL) and vitamin K2 (MK-4 and MK-7), respectively. The limits of detection and limits of quantification for the target nutritional compounds ranged between 0.04-0.05 µg/mL, respectively. The validation results indicated that the method had reasonable linearity (
Identifiants
pubmed: 34834074
pii: molecules26226982
doi: 10.3390/molecules26226982
pmc: PMC8624398
pii:
doi:
Substances chimiques
Vitamin K 2
11032-49-8
Cholecalciferol
1C6V77QF41
menatetrenone
27Y876D139
menaquinone 7
8427BML8NY
Calcitriol
FXC9231JVH
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : CCCDI-UEFISCDI, PNCDI III
ID : PN-III-P2-2.1-PED-2019-3373
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