Pulmonary surfactant inactivation by β-D-glucan and protective role of surfactant protein A.
Lung
Membrane fluidity
Monolayers
Phosphatidylcholine
SP-A
Surfactant inhibition
Journal
Colloids and surfaces. B, Biointerfaces
ISSN: 1873-4367
Titre abrégé: Colloids Surf B Biointerfaces
Pays: Netherlands
ID NLM: 9315133
Informations de publication
Date de publication:
Feb 2022
Feb 2022
Historique:
received:
28
05
2021
revised:
10
11
2021
accepted:
16
11
2021
pubmed:
28
11
2021
medline:
15
1
2022
entrez:
27
11
2021
Statut:
ppublish
Résumé
Pulmonary fungal infections lead to damage of the endogenous lung surfactant system. However, the molecular mechanism underlying surfactant inhibition is unknown. β-D-glucan is the major component of pathogenic fungal cell walls and is also present in organic dust, which increases the risk of respiratory diseases. The objective of this study was to characterize the interaction of this D-glucopyranose polymer with pulmonary surfactant. Our results show that β-D-glucan induced a concentration-dependent inhibition of the surface adsorption, respreading, and surface tension-lowering activity of surfactant preparations containing surfactant proteins SP-B and SP-C. Our data support a new mechanism of surfactant inhibition that consists in the extraction of phospholipid molecules from surfactant membranes by β-D-glucan. As a result, surfactant membranes became more fluid, as demonstrated by fluorescence anisotropy, and showed decreased T
Identifiants
pubmed: 34836708
pii: S0927-7765(21)00683-4
doi: 10.1016/j.colsurfb.2021.112237
pii:
doi:
Substances chimiques
Glucans
0
Phospholipids
0
Pulmonary Surfactant-Associated Protein A
0
Pulmonary Surfactant-Associated Protein B
0
Pulmonary Surfactants
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
112237Informations de copyright
Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.