A temperature-sensitive metabolic valve and a transcriptional feedback loop drive rapid homeoviscous adaptation in Escherichia coli.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
30 Oct 2024
30 Oct 2024
Historique:
received:
18
09
2024
accepted:
17
10
2024
medline:
31
10
2024
pubmed:
31
10
2024
entrez:
31
10
2024
Statut:
epublish
Résumé
All free-living microorganisms homeostatically maintain the fluidity of their membranes by adapting lipid composition to environmental temperatures. Here, we quantify enzymes and metabolic intermediates of the Escherichia coli fatty acid and phospholipid synthesis pathways, to describe how this organism measures temperature and restores optimal membrane fluidity within a single generation after a temperature shock. A first element of this regulatory system is a temperature-sensitive metabolic valve that allocates flux between the saturated and unsaturated fatty acid synthesis pathways via the branchpoint enzymes FabI and FabB. A second element is a transcription-based negative feedback loop that counteracts the temperature-sensitive valve. The combination of these elements accelerates membrane adaptation by causing a transient overshoot in the synthesis of saturated or unsaturated fatty acids following temperature shocks. This strategy is comparable to increasing the temperature of a water bath by adding water that is excessively hot rather than adding water at the desired temperature. These properties are captured in a mathematical model, which we use to show how hard-wired parameters calibrate the system to generate membrane compositions that maintain constant fluidity across temperatures. We hypothesize that core features of the E. coli system will prove to be ubiquitous features of homeoviscous adaptation systems.
Identifiants
pubmed: 39477942
doi: 10.1038/s41467-024-53677-5
pii: 10.1038/s41467-024-53677-5
doi:
Substances chimiques
Escherichia coli Proteins
0
Fatty Acids
0
Phospholipids
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
9386Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM095970
Pays : United States
Organisme : NIA NIH HHS
ID : R21 AG073807
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM095970
Pays : United States
Organisme : NIA NIH HHS
ID : R21 AG073807
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM095970
Pays : United States
Organisme : NIA NIH HHS
ID : R21 AG073807
Pays : United States
Informations de copyright
© 2024. The Author(s).
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