A healthy Bifidobacterium dentium caramel cocktail.

Bifidobacterium Crystallography, X-Ray Glycoside Hydrolases / chemistry
CAZy, channel GH172, caramel Glycoside hydrolase difructo anhydride enzyme mechanism molecular dynamics prebiotic protein crystallography

Journal

The Journal of biological chemistry
ISSN: 1083-351X
Titre abrégé: J Biol Chem
Pays: United States
ID NLM: 2985121R

Informations de publication

Date de publication:
01 2022
Historique:
accepted: 22 11 2021
pubmed: 29 11 2021
medline: 29 4 2022
entrez: 28 11 2021
Statut: ppublish

Résumé

β-d-fructofuranosyl glycosidases are enzymes that produce health-beneficial fructooligosaccharides from natural fructans. In a recent issue of JBC, Kashima et al. identified a novel α-d-fructofuranosyl-active enzyme, αFFase1, from the caries-associated bacterium Bifidobacterium dentium. αFFase1 reversibly forms a potential prebiotic also found in caramel, difructose dianhydride I, via intramolecular condensation of the substrate inulobiose. Kashima et al. elegantly combine NMR, X-ray crystallography, and molecular dynamics to describe an original mechanism for the reversible reactions catalyzed by αFFase1 that establishes the new glycoside hydrolase family GH172.

Identifiants

DOI: 10.1016/j.jbc.2021.101452 PMID: 34838821 PMC: PMC8718956
pubmed: 34838821
pii: S0021-9258(21)01261-8
doi: 10.1016/j.jbc.2021.101452
pmc: PMC8718956
pii:
doi:

Substances chimiques

Glycoside Hydrolases EC 3.2.1.-

Types de publication

Journal Article Research Support, Non-U.S. Gov't Comment

Langues

eng

Sous-ensembles de citation

IM

Pagination

101452

Commentaires et corrections

Type : CommentOn

Informations de copyright

Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Conflict of interest The authors declare that they have no conflicts of interest with the content of this article.

Auteurs

David Teze (D)

Enzyme Engineering and Structural Biology, The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kgs. Lyngby, Denmark. Electronic address: datez@biosustain.dtu.dk.

Birte Svensson (B)

Enzyme and Protein Chemistry, Department of Biotechnology and Biomedicine, Technical University of Denmark, Kgs. Lyngby, Denmark. Electronic address: bis@bio.dtu.dk.

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Classifications MeSH

questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Bâtonnets à Gram positif › Bâtonnets asporogènes à Gram positif › Bâtonnets asporogènes irréguliers à Gram positif › Actinobacteria › Bifidobacterium › A healthy Bifidobacterium dentium caramel cocktail.
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Cristallographie aux rayons X › A healthy Bifidobacterium dentium caramel cocktail.
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Hydrolases › Glycosidases › A healthy Bifidobacterium dentium caramel cocktail.