Deletion of pancreas-specific miR-216a reduces beta-cell mass and inhibits pancreatic cancer progression in mice.


Journal

Cell reports. Medicine
ISSN: 2666-3791
Titre abrégé: Cell Rep Med
Pays: United States
ID NLM: 101766894

Informations de publication

Date de publication:
16 11 2021
Historique:
received: 11 12 2019
revised: 08 03 2021
accepted: 05 10 2021
entrez: 29 11 2021
pubmed: 30 11 2021
medline: 30 11 2021
Statut: epublish

Résumé

miRNAs have crucial functions in many biological processes and are candidate biomarkers of disease. Here, we show that miR-216a is a conserved, pancreas-specific miRNA with important roles in pancreatic islet and acinar cells. Deletion of miR-216a in mice leads to a reduction in islet size, β-cell mass, and insulin levels. Single-cell RNA sequencing reveals a subpopulation of β-cells with upregulated acinar cell markers under a high-fat diet. miR-216a is induced by TGF-β signaling, and inhibition of miR-216a increases apoptosis and decreases cell proliferation in pancreatic cells. Deletion of miR-216a in the pancreatic cancer-prone mouse line

Identifiants

pubmed: 34841287
doi: 10.1016/j.xcrm.2021.100434
pii: S2666-3791(21)00296-2
pmc: PMC8606901
doi:

Substances chimiques

MIRN216 microRNA, mouse 0
MicroRNAs 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Pagination

100434

Subventions

Organisme : CIHR
Pays : Canada

Informations de copyright

© 2021 The Author(s).

Déclaration de conflit d'intérêts

The authors declare no competing interests.

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Auteurs

Suheda Erener (S)

Department of Cellular & Physiological Sciences, Life Sciences Institute, University of British Columbia, Vancouver, BC, Canada.
Institute for Diabetes and Cancer, Helmholtz Center Munich, Neuherberg, Germany.

Cara E Ellis (CE)

Department of Cellular & Physiological Sciences, Life Sciences Institute, University of British Columbia, Vancouver, BC, Canada.

Adam Ramzy (A)

Department of Cellular & Physiological Sciences, Life Sciences Institute, University of British Columbia, Vancouver, BC, Canada.

Maria M Glavas (MM)

Department of Cellular & Physiological Sciences, Life Sciences Institute, University of British Columbia, Vancouver, BC, Canada.

Shannon O'Dwyer (S)

Department of Cellular & Physiological Sciences, Life Sciences Institute, University of British Columbia, Vancouver, BC, Canada.

Sandra Pereira (S)

Department of Cellular & Physiological Sciences, Life Sciences Institute, University of British Columbia, Vancouver, BC, Canada.

Tom Wang (T)

Department of Cellular & Physiological Sciences, Life Sciences Institute, University of British Columbia, Vancouver, BC, Canada.

Janice Pang (J)

Department of Cellular & Physiological Sciences, Life Sciences Institute, University of British Columbia, Vancouver, BC, Canada.

Jennifer E Bruin (JE)

Department of Cellular & Physiological Sciences, Life Sciences Institute, University of British Columbia, Vancouver, BC, Canada.
Department of Biology and Institute of Biochemistry, Carleton University, Ottawa, ON, Canada.

Michael J Riedel (MJ)

Department of Cellular & Physiological Sciences, Life Sciences Institute, University of British Columbia, Vancouver, BC, Canada.

Robert K Baker (RK)

Department of Cellular & Physiological Sciences, Life Sciences Institute, University of British Columbia, Vancouver, BC, Canada.

Travis D Webber (TD)

Department of Cellular & Physiological Sciences, Life Sciences Institute, University of British Columbia, Vancouver, BC, Canada.

Marina Lesina (M)

Comprehensive Cancer Center Munich, Technical University of Munich, Munich, Germany.

Matthias Blüher (M)

Helmholtz Institute for Metabolic, Obesity and Vascular Research (HI-MAG) of the Helmholtz Zentrum München at the University of Leipzig and University Hospital Leipzig, Leipzig, Germany.
Medical Department III - Endocrinology, Nephrology, Rheumatology, University of Leipzig Medical Center, Leipzig, Germany.

Hana Algül (H)

Comprehensive Cancer Center Munich, Technical University of Munich, Munich, Germany.

Janel L Kopp (JL)

Department of Cellular & Physiological Sciences, Life Sciences Institute, University of British Columbia, Vancouver, BC, Canada.

Stephan Herzig (S)

Institute for Diabetes and Cancer, Helmholtz Center Munich, Neuherberg, Germany.
Joint Heidelberg-IDC Translational Diabetes Program, Inner Medicine 1, Heidelberg University Hospital, Heidelberg, Germany.
Technical University Munich, 85764 Neuherberg, Germany.
Deutsches Zentrum für Diabetesforschung, 85764 Neuherberg, Germany.

Timothy J Kieffer (TJ)

Department of Cellular & Physiological Sciences, Life Sciences Institute, University of British Columbia, Vancouver, BC, Canada.
Department of Surgery, University of British Columbia, Vancouver, BC, Canada.
School of Biomedical Engineering, University of British Columbia, Vancouver, BC, Canada.

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