Rapid Identification of SARS-CoV-2 Variants of Concern Using a Portable

COVID-19 / epidemiology COVID-19 Nucleic Acid Testing Equatorial Guinea / epidemiology Gene Deletion Humans Mutation Polymorphism, Single Nucleotide SARS-CoV-2 / classification

Journal

Analytical chemistry
ISSN: 1520-6882
Titre abrégé: Anal Chem
Pays: United States
ID NLM: 0370536

Informations de publication

Date de publication:
14 12 2021
Historique:
pubmed: 3 12 2021
medline: 21 12 2021
entrez: 2 12 2021
Statut: ppublish

Résumé

The need for tools that facilitate rapid detection and continuous monitoring of SARS-CoV-2 variants of concern (VOCs) is greater than ever, as these variants are more transmissible and therefore increase the pressure of COVID-19 on healthcare systems. To address this demand, we aimed at developing and evaluating a robust and fast diagnostic approach for the identification of SARS-CoV-2 VOC-associated spike gene mutations. Our diagnostic assays detect the E484K and N501Y single-nucleotide polymorphisms (SNPs) as well as a spike gene deletion (HV69/70) and can be run on standard laboratory equipment or on the portable rapid diagnostic technology platform

Identifiants

DOI: 10.1021/acs.analchem.1c02368 PMID: 34852455
pubmed: 34852455
doi: 10.1021/acs.analchem.1c02368
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

16350-16359

Auteurs

Philippe Bechtold (P)

Institute for Chemical and Bioengineering, ETH Zurich, 8093 Zuerich, Switzerland.
Diaxxo AG, 8093 Zuerich, Switzerland.

Philipp Wagner (P)

Swiss Tropical and Public Health Institute, 4051 Basel, Switzerland.
University of Basel, 4051 Basel, Switzerland.

Salome Hosch (S)

Swiss Tropical and Public Health Institute, 4051 Basel, Switzerland.
University of Basel, 4051 Basel, Switzerland.

Denise Siegrist (D)

Spiez Laboratory, 3700 Spiez, Switzerland.

Amalia Ruiz-Serrano (A)

Institute for Chemical and Bioengineering, ETH Zurich, 8093 Zuerich, Switzerland.
Diaxxo AG, 8093 Zuerich, Switzerland.

Michele Gregorini (M)

Institute for Chemical and Bioengineering, ETH Zurich, 8093 Zuerich, Switzerland.
Diaxxo AG, 8093 Zuerich, Switzerland.

Maxmillian Mpina (M)

Swiss Tropical and Public Health Institute, 4051 Basel, Switzerland.
University of Basel, 4051 Basel, Switzerland.
Laboratorio de Investigaciones de Baney, P.O. Box 338, Baney, Equatorial Guinea.

Florentino Abaga Ondó (FA)

Ministry of Health and Social Welfare, P.O. Box 209, Malabo, Equatorial Guinea.

Justino Obama (J)

Ministry of Health and Social Welfare, P.O. Box 209, Malabo, Equatorial Guinea.

Mitoha Ondo'o Ayekaba (MO)

Ministry of Health and Social Welfare, P.O. Box 209, Malabo, Equatorial Guinea.

Olivier Engler (O)

Spiez Laboratory, 3700 Spiez, Switzerland.

Wendelin J Stark (WJ)

Institute for Chemical and Bioengineering, ETH Zurich, 8093 Zuerich, Switzerland.
Diaxxo AG, 8093 Zuerich, Switzerland.
ORCID: 0000-0002-8957-7687

Claudia A Daubenberger (CA)

Swiss Tropical and Public Health Institute, 4051 Basel, Switzerland.
University of Basel, 4051 Basel, Switzerland.

Tobias Schindler (T)

Swiss Tropical and Public Health Institute, 4051 Basel, Switzerland.
University of Basel, 4051 Basel, Switzerland.
ORCID: 0000-0002-5961-095X

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Classifications MeSH

questionsmedicales.fr › Maladies de l'appareil respiratoire › Infections de l'appareil respiratoire › Pneumopathie infectieuse › Pneumopathie virale › COVID-19 › Rapid Identification of SARS-CoV-2 Variants of...
questionsmedicales.fr › Techniques d'investigation › Techniques de laboratoire clinique › Dépistage de la COVID-19 › Détection de l'acide nucléique du virus de la COVID-19 › Rapid Identification of SARS-CoV-2 Variants of...
questionsmedicales.fr › Régions géographiques › Afrique › Afrique subsaharienne › Afrique centrale › Guinée équatoriale › Rapid Identification of SARS-CoV-2 Variants of...
questionsmedicales.fr › Phénomènes génétiques › Mutagenèse › Délétion de séquence › Délétion de gène › Rapid Identification of SARS-CoV-2 Variants of...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Rapid Identification of SARS-CoV-2 Variants of...
questionsmedicales.fr › Phénomènes génétiques › Variation génétique › Mutation › Rapid Identification of SARS-CoV-2 Variants of...
questionsmedicales.fr › Phénomènes génétiques › Variation génétique › Polymorphisme génétique › Polymorphisme de nucléotide simple › Rapid Identification of SARS-CoV-2 Variants of...
questionsmedicales.fr › Virus › Virus à ARN › Virus à ARN à brin positif › Nidovirales › Coronaviridae › Coronavirus › Betacoronavirus › Virus du SRAS › SARS-CoV-2 › Rapid Identification of SARS-CoV-2 Variants of...