Highly efficient, genotype-independent transformation and gene editing in watermelon (Citrullus lanatus) using a chimeric ClGRF4-GIF1 gene.
GIF
GRF
gene editing
genetic transforamtion
watermelon
Journal
Journal of integrative plant biology
ISSN: 1744-7909
Titre abrégé: J Integr Plant Biol
Pays: China (Republic : 1949- )
ID NLM: 101250502
Informations de publication
Date de publication:
Dec 2021
Dec 2021
Historique:
received:
29
11
2021
accepted:
01
12
2021
pubmed:
5
12
2021
medline:
5
4
2022
entrez:
4
12
2021
Statut:
ppublish
Résumé
Efficient genetic transformation has the potential to advance research and breeding in watermelon (Citrullus lanatus), but regeneration from tissue culture remains challenging. Previous work showed that expressing a fusion of two interacting transcription factors, GROWTH-REGULATING FACTOR4 (GRF4) and GRF-INTERACTING FACTOR1 (GIF1), improved regeneration in wheat (Triticum aestivum). By overexpressing a chimeric fusion of ClGRF4 and ClGIF1, we achieved highly efficient transformation in watermelon. Mutating the mi396 microRNA target site in ClGRF further boosted the transformation efficiency up to 67.27% in a genotype-independent manner. ClGRF4-GIF1 can also be combined with clustered regularly interspaced palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) genome editing tools to achieve highly efficient gene editing in watermelon, which we used to successfully create diploid seedless watermelon. This research thus puts forward a powerful transformation tool for future watermelon research and breeding.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2038-2042Subventions
Organisme : National Youth Talent Program
ID : A279021801
Organisme : Fundamental Research Fund from Northwest A&F University
ID : Z1090221008
Organisme : Key R&D Project from Yangling Seed Industry Innovation Center
ID : 2021
Informations de copyright
© 2021 Institute of Botany, Chinese Academy of Sciences.
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