Extracellular vesicles and pasteurized cells derived from Akkermansia muciniphila protect against high-fat induced obesity in mice.
Akkermansia muciniphila
Extracellular vesicles
Obesity
Pasteurization
Journal
Microbial cell factories
ISSN: 1475-2859
Titre abrégé: Microb Cell Fact
Pays: England
ID NLM: 101139812
Informations de publication
Date de publication:
04 Dec 2021
04 Dec 2021
Historique:
received:
29
07
2021
accepted:
16
11
2021
entrez:
5
12
2021
pubmed:
6
12
2021
medline:
19
2
2022
Statut:
epublish
Résumé
Several studies have shown that probiotics have beneficial effects on weight control and metabolic health. In addition to probiotics, recent studies have investigated the effects of paraprobiotics and postbiotics. Therefore, we evaluated the preventive effects of live and pasteurized Akkermansia muciniphila MucT (A. muciniphila) and its extracellular vesicles (EVs) on HFD-induced obesity. The results showed that body weight, metabolic tissues weight, food consumption, and plasma metabolic parameters were increased in the HFD group, whereas A. muciniphila preventive treatments inhibited these HFD. The effects of pasteurized A. muciniphila and its extracellular vesicles were more noticeable than its active form. The HFD led to an increase in the colonic, adipose tissue, and liver inflammations and increased the expression of genes involved in lipid metabolism and homeostasis. Nevertheless, these effects were inhibited in mice that were administered A. muciniphila and its EVs. The assessment of the gut microbiota revealed significant differences in the microbiota composition after feeding with HFD. However, all treatments restored the alterations in some bacterial genera and closely resemble the control group. Also, the correlation analysis indicated that some gut microbiota might be associated with obesity-related indices. Pasteurized A. muciniphila and its EVs, as paraprobiotic and postbiotic agents, were found to play a key role in the regulation of metabolic functions to prevent obesity, probably by affecting the gut-adipose-liver axis.
Sections du résumé
BACKGROUND
BACKGROUND
Several studies have shown that probiotics have beneficial effects on weight control and metabolic health. In addition to probiotics, recent studies have investigated the effects of paraprobiotics and postbiotics. Therefore, we evaluated the preventive effects of live and pasteurized Akkermansia muciniphila MucT (A. muciniphila) and its extracellular vesicles (EVs) on HFD-induced obesity.
RESULTS
RESULTS
The results showed that body weight, metabolic tissues weight, food consumption, and plasma metabolic parameters were increased in the HFD group, whereas A. muciniphila preventive treatments inhibited these HFD. The effects of pasteurized A. muciniphila and its extracellular vesicles were more noticeable than its active form. The HFD led to an increase in the colonic, adipose tissue, and liver inflammations and increased the expression of genes involved in lipid metabolism and homeostasis. Nevertheless, these effects were inhibited in mice that were administered A. muciniphila and its EVs. The assessment of the gut microbiota revealed significant differences in the microbiota composition after feeding with HFD. However, all treatments restored the alterations in some bacterial genera and closely resemble the control group. Also, the correlation analysis indicated that some gut microbiota might be associated with obesity-related indices.
CONCLUSIONS
CONCLUSIONS
Pasteurized A. muciniphila and its EVs, as paraprobiotic and postbiotic agents, were found to play a key role in the regulation of metabolic functions to prevent obesity, probably by affecting the gut-adipose-liver axis.
Identifiants
pubmed: 34863163
doi: 10.1186/s12934-021-01709-w
pii: 10.1186/s12934-021-01709-w
pmc: PMC8645101
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
219Informations de copyright
© 2021. The Author(s).
Références
Biochem Soc Trans. 2018 Oct 19;46(5):1021-1027
pubmed: 30154095
Nat Med. 2019 Jul;25(7):1096-1103
pubmed: 31263284
Biochem Pharmacol. 2016 Nov 1;119:8-16
pubmed: 27179976
Nutrition. 2018 Oct;54:40-47
pubmed: 29705500
BMC Microbiol. 2017 May 22;17(1):120
pubmed: 28532414
PLoS One. 2015 Oct 06;10(10):e0138880
pubmed: 26439630
J Endocrinol. 2018 May;237(2):87-100
pubmed: 29507043
Nutrients. 2019 Jan 24;11(2):
pubmed: 30678355
Proc Natl Acad Sci U S A. 2013 May 28;110(22):9066-71
pubmed: 23671105
Nat Rev Immunol. 2020 Jan;20(1):40-54
pubmed: 31388093
Diabetologia. 2007 Nov;50(11):2374-83
pubmed: 17823788
Curr Hypertens Rep. 2018 Feb 26;20(2):12
pubmed: 29480368
Nat Med. 2017 Jan;23(1):107-113
pubmed: 27892954
Nutr Diabetes. 2014 Jun 30;4:e122
pubmed: 24979151
Front Microbiol. 2017 Sep 26;8:1804
pubmed: 29033903
Nature. 2006 Dec 21;444(7122):1027-31
pubmed: 17183312
Nat Rev Microbiol. 2013 Sep;11(9):639-47
pubmed: 23912213
Eur J Nutr. 2019 Feb;58(1):27-43
pubmed: 30043184
Food Funct. 2016 Dec 7;7(12):4869-4879
pubmed: 27812583
Adv Nutr. 2019 Jan 1;10(suppl_1):S49-S66
pubmed: 30721959
Gastroenterology. 2020 May;158(7):1899-1912
pubmed: 32061598
Nutr Rev. 2015 Jun;73(6):376-85
pubmed: 26011912
Gut Microbes. 2020 Sep 2;11(5):1231-1245
pubmed: 32167023
ISME J. 2017 Jul;11(7):1667-1679
pubmed: 28375212
Exp Mol Med. 2018 Feb 23;50(2):e450
pubmed: 29472701
Sci Rep. 2015 Nov 02;5:15970
pubmed: 26522240
Appl Environ Microbiol. 2015 Oct;81(19):6749-56
pubmed: 26187967
Appl Environ Microbiol. 2020 Mar 18;86(7):
pubmed: 31953338
BMC Gastroenterol. 2014 Nov 18;14:189
pubmed: 25407511
Probiotics Antimicrob Proteins. 2019 Mar;11(1):175-185
pubmed: 29353414
Obes Surg. 2019 May;29(5):1663-1674
pubmed: 30793228
PLoS One. 2017 Mar 1;12(3):e0173004
pubmed: 28249045
Lipids Health Dis. 2020 Feb 7;19(1):20
pubmed: 32028957
PLoS One. 2012;7(8):e42529
pubmed: 22880019
Microb Pathog. 2020 Jul;144:104200
pubmed: 32289465
Chem Biol Interact. 2020 Oct 1;330:109199
pubmed: 32805210
Appetite. 2006 Jan;46(1):6-10
pubmed: 16229924
Gut. 2014 May;63(5):727-35
pubmed: 23804561
Nature. 2006 Dec 21;444(7122):1022-3
pubmed: 17183309
Sci Rep. 2019 May 2;9(1):6821
pubmed: 31048785
Front Immunol. 2019 Dec 11;10:2800
pubmed: 31921106
Int J Mol Sci. 2019 May 13;20(9):
pubmed: 31085992
Curr Opin Clin Nutr Metab Care. 2007 Nov;10(6):729-34
pubmed: 18089955
Clin Chim Acta. 2020 Apr;503:19-34
pubmed: 31923423
Sci Rep. 2021 Sep 9;11(1):17898
pubmed: 34504116
J Endocr Soc. 2020 Feb 20;4(2):bvz039
pubmed: 32099951
Cell Host Microbe. 2008 Apr 17;3(4):213-23
pubmed: 18407065
Nature. 2009 Jan 22;457(7228):480-4
pubmed: 19043404
J Biol Chem. 2015 Feb 20;290(8):5007-5014
pubmed: 25540199
Nat Commun. 2019 Jan 28;10(1):457
pubmed: 30692526
Nutrients. 2020 May 19;12(5):
pubmed: 32438689
Curr Obes Rep. 2020 Sep;9(3):179-192
pubmed: 32472285
Trends Endocrinol Metab. 2014 Mar;25(3):146-55
pubmed: 24397894
Food Funct. 2016 May 18;7(5):2374-88
pubmed: 27163114
Obesity (Silver Spring). 2013 Dec;21(12):E607-15
pubmed: 23526699
Lancet. 2014 Aug 30;384(9945):766-81
pubmed: 24880830
PeerJ. 2017 Jan 3;5:e2840
pubmed: 28070463
Microbiol Spectr. 2021 Oct 31;9(2):e0048421
pubmed: 34549998
Front Microbiol. 2019 Oct 01;10:2155
pubmed: 31632356
Diabetologia. 2017 Apr;60(4):613-617
pubmed: 28013341
PLoS One. 2013 Oct 24;8(10):e76520
pubmed: 24204633
PLoS One. 2013;8(3):e59470
pubmed: 23555678
Proc Natl Acad Sci U S A. 2005 Aug 2;102(31):11070-5
pubmed: 16033867
Microb Biotechnol. 2018 Sep;11(5):859-868
pubmed: 29856120
Molecules. 2020 Mar 25;25(7):
pubmed: 32218367
Probiotics Antimicrob Proteins. 2021 Jun;13(3):776-787
pubmed: 33433897
Cell Metab. 2010 Dec 1;12(6):580-92
pubmed: 21109191
Microb Cell Fact. 2020 Aug 20;19(1):168
pubmed: 32819443
Gastroenterol Hepatol Bed Bench. 2019 Spring;12(2):163-168
pubmed: 31191842
J Nutr Biochem. 2019 Feb;64:88-100
pubmed: 30471564
Int J Syst Evol Microbiol. 2004 Sep;54(Pt 5):1469-1476
pubmed: 15388697
Diabetes Obes Metab. 2016 Jun;18(6):558-70
pubmed: 26936802
J Gastrointestin Liver Dis. 2018 Mar;27(1):41-49
pubmed: 29557414