Low-cost 3D-printed inverted microscope to detect Mycobacterium tuberculosis in a MODS culture.
3D printing
Diagnostics
MODS
Microscopy
Tuberculosis
Journal
Tuberculosis (Edinburgh, Scotland)
ISSN: 1873-281X
Titre abrégé: Tuberculosis (Edinb)
Pays: Scotland
ID NLM: 100971555
Informations de publication
Date de publication:
01 2022
01 2022
Historique:
received:
05
10
2021
revised:
26
11
2021
accepted:
27
11
2021
pubmed:
6
12
2021
medline:
11
3
2022
entrez:
5
12
2021
Statut:
ppublish
Résumé
MODS, an assay for diagnosis of tuberculosis and drug-susceptibility, is based in the microscopic observation of the characteristic cords of Mycobacterium tuberculosis colonies grown in liquid media. An inverted optical microscope (100× magnification) is required to observe and interpret MODS cultures. Unfortunately, the cost of commercial inverted microscopes is not affordable in low resource settings. To perform a diagnosis of tuberculosis using the MODS assay, images with modest quality are enough for proper interpretation. Therefore, the use of a high cost commercial inverted optical microscope is not indispensable. In this study, we designed a prototype of an optical inverted microscope created by 3D-printing and based on a smartphone. The system was evaluated with 226 MODS TB positive and 207 MODS TB negative digital images. These images were obtained from 10 sputum samples MODS positive and 10 sputum samples MODS negative. The quality of all images was assessed by a qualified technician, in terms of adequacy to interpret and classify them as positive or negative for tuberculosis. The quality of the images was considered appropriate for MODS interpretation. All the 20 samples were correctly classified (as TB positive/negative) by reading with the prototype 3D-printed inverted microscope.
Identifiants
pubmed: 34864388
pii: S1472-9792(21)00108-6
doi: 10.1016/j.tube.2021.102158
pmc: PMC8818028
mid: NIHMS1761412
pii:
doi:
Substances chimiques
Antitubercular Agents
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
102158Subventions
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : FIC NIH HHS
ID : R25 TW009720
Pays : United States
Organisme : Wellcome Trust
ID : 099805/Z/112/Z
Pays : United Kingdom
Informations de copyright
Copyright © 2021 Elsevier Ltd. All rights reserved.
Références
PLoS One. 2019 Feb 27;14(2):e0212094
pubmed: 30811445
PLoS One. 2016 Apr 06;11(4):e0152023
pubmed: 27050661
PLoS One. 2010 Mar 23;5(3):e9577
pubmed: 20351778
J Clin Microbiol. 2004 Oct;42(10):4432-7
pubmed: 15472289
Trans R Soc Trop Med Hyg. 2009 Jun;103(6):638-40
pubmed: 19036392
N Engl J Med. 2006 Oct 12;355(15):1539-50
pubmed: 17035648
J Microsc. 2011 Jun;242(3):325-30
pubmed: 21250995
PLoS One. 2013 Dec 16;8(12):e82809
pubmed: 24358227
J Clin Microbiol. 2000 Mar;38(3):1203-8
pubmed: 10699023
Eur Respir J. 2012 Jun;39(6):1511-9
pubmed: 22005918