Shelf-Life Extension of Fc-Fused Single Chain Fragment Variable Antibodies by Lyophilization.
IgG
antibody formatting
antibody stability
freeze-drying
lyophilization
phage display
recombinant antibody
scFv-Fc
Journal
Frontiers in cellular and infection microbiology
ISSN: 2235-2988
Titre abrégé: Front Cell Infect Microbiol
Pays: Switzerland
ID NLM: 101585359
Informations de publication
Date de publication:
2021
2021
Historique:
received:
29
08
2021
accepted:
25
10
2021
entrez:
6
12
2021
pubmed:
7
12
2021
medline:
28
1
2022
Statut:
epublish
Résumé
Generation of sequence defined antibodies from universal libraries by phage display has been established over the past three decades as a robust method to cope with the increasing market demand in therapy, diagnostics and research. For applications requiring the bivalent antigen binding and an Fc part for detection, phage display generated single chain Fv (scFv) antibody fragments can rapidly be genetically fused to the Fc moiety of an IgG for the production in eukaryotic cells of antibodies with IgG-like properties. In contrast to conversion of scFv into IgG format, the conversion to scFv-Fc requires only a single cloning step, and provides significantly higher yields in transient cell culture production than IgG. ScFv-Fcs can be effective as neutralizing antibodies
Identifiants
pubmed: 34869052
doi: 10.3389/fcimb.2021.717689
pmc: PMC8634725
doi:
Substances chimiques
Antibodies, Neutralizing
0
Single-Chain Antibodies
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
717689Informations de copyright
Copyright © 2021 Schneider, Kirmann, Wenzel, Grosch, Polten, Meier, Becker, Matejtschuk, Hust, Russo and Dübel.
Déclaration de conflit d'intérêts
GR, EW, and SD are co-founders and shareholders of Abcalis GmbH. SD and MH are co-founders and shareholders of mAb-factory GmbH, YUMAB GmbH and NordenVaccines GmbH. DM is co-founder and shareholder of mAb-factory GmbH. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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