A genetically encoded fluorescent biosensor for extracellular L-lactate.
Bacterial Proteins
/ genetics
Binding Sites
/ genetics
Biosensing Techniques
/ methods
Cell Line, Tumor
Crystallography, X-Ray
Fluorescence
Green Fluorescent Proteins
/ chemistry
HEK293 Cells
HeLa Cells
Humans
Lactic Acid
/ analysis
Microscopy, Fluorescence
Periplasmic Proteins
/ genetics
Protein Binding
Recombinant Fusion Proteins
/ chemistry
Reproducibility of Results
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
06 12 2021
06 12 2021
Historique:
received:
10
06
2021
accepted:
15
11
2021
entrez:
7
12
2021
pubmed:
8
12
2021
medline:
11
1
2022
Statut:
epublish
Résumé
L-Lactate, traditionally considered a metabolic waste product, is increasingly recognized as an important intercellular energy currency in mammals. To enable investigations of the emerging roles of intercellular shuttling of L-lactate, we now report an intensiometric green fluorescent genetically encoded biosensor for extracellular L-lactate. This biosensor, designated eLACCO1.1, enables cellular resolution imaging of extracellular L-lactate in cultured mammalian cells and brain tissue.
Identifiants
pubmed: 34873165
doi: 10.1038/s41467-021-27332-2
pii: 10.1038/s41467-021-27332-2
pmc: PMC8648760
doi:
Substances chimiques
Bacterial Proteins
0
Periplasmic Proteins
0
Recombinant Fusion Proteins
0
Green Fluorescent Proteins
147336-22-9
Lactic Acid
33X04XA5AT
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
7058Subventions
Organisme : NINDS NIH HHS
ID : F31 NS108593
Pays : United States
Organisme : NINDS NIH HHS
ID : U01 NS094246
Pays : United States
Organisme : NINDS NIH HHS
ID : U24 NS109107
Pays : United States
Organisme : CIHR
ID : FS-154310
Pays : Canada
Informations de copyright
© 2021. The Author(s).
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