Epididymis incision as a method to collect epididymal sperm cells in alpacas.
Vicugna pacos
alpaca
epididymis
extraction method
sperm recovery
Journal
Veterinary medicine and science
ISSN: 2053-1095
Titre abrégé: Vet Med Sci
Pays: England
ID NLM: 101678837
Informations de publication
Date de publication:
01 2022
01 2022
Historique:
pubmed:
8
12
2021
medline:
1
3
2022
entrez:
7
12
2021
Statut:
ppublish
Résumé
Epididymal sperm can be collected post-castration or post-mortem. This method has already been described for alpaca (Vicugna pacos), but little data are available on success rate and the influence of diluent on it. The aim was to investigate the effectiveness of epididymis incision as an extraction method. Post-castration epididymides (n = 78) were incised and semen was collected from cut surfaces. Further sperm were flushed with two semen extenders. For the left epididymis, a diluent without animal proteins and for the right, a diluent with egg yolk was utilised. Collected sperms were immediately spermatologically examined. Due to incorrect measurements, the samples of seven epididymides were not analysed. An evaluation was possible in 58 samples. Average density was 108.80 ± 83.28 million/mL and motility was 53.30 ± 18.17%. On average, 76.70 ± 11.60% of the sperm were vital in eosin-stained specimens. In the hypoosmotic swelling test, an average of 69.50 ± 10.48% of the sperm had an intact plasma membrane. Semen extender had no effect on spermatological parameters (p > 0.05). Overall success rate of sperm recovery was 83%. Extraction of epididymal sperm was possible in 68.80% of the 2-year-old males. The 13 epididymides from which no sperm recovery was possible were histologically examined and for 10, there was no histological evidence of sperm. The corrected success rate was 95.60%. Testicular volume (p = 0.0453), but not age (p = 0.62), had an effect on the probability of obtaining sperm.
Sections du résumé
BACKGROUND
Epididymal sperm can be collected post-castration or post-mortem. This method has already been described for alpaca (Vicugna pacos), but little data are available on success rate and the influence of diluent on it.
OBJECTIVE
The aim was to investigate the effectiveness of epididymis incision as an extraction method.
METHOD
Post-castration epididymides (n = 78) were incised and semen was collected from cut surfaces. Further sperm were flushed with two semen extenders. For the left epididymis, a diluent without animal proteins and for the right, a diluent with egg yolk was utilised. Collected sperms were immediately spermatologically examined.
RESULTS
Due to incorrect measurements, the samples of seven epididymides were not analysed. An evaluation was possible in 58 samples. Average density was 108.80 ± 83.28 million/mL and motility was 53.30 ± 18.17%. On average, 76.70 ± 11.60% of the sperm were vital in eosin-stained specimens. In the hypoosmotic swelling test, an average of 69.50 ± 10.48% of the sperm had an intact plasma membrane. Semen extender had no effect on spermatological parameters (p > 0.05). Overall success rate of sperm recovery was 83%. Extraction of epididymal sperm was possible in 68.80% of the 2-year-old males. The 13 epididymides from which no sperm recovery was possible were histologically examined and for 10, there was no histological evidence of sperm. The corrected success rate was 95.60%.
CONCLUSION
Testicular volume (p = 0.0453), but not age (p = 0.62), had an effect on the probability of obtaining sperm.
Identifiants
pubmed: 34874129
doi: 10.1002/vms3.681
pmc: PMC8788956
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
157-163Informations de copyright
© 2021 The Authors. Veterinary Medicine and Science published by John Wiley & Sons Ltd.
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Vet Med Sci. 2022 Jan;8(1):157-163
pubmed: 34874129