RT-QuIC detection of pathological prion protein in subclinical goats following experimental oral transmission of L-type BSE.
Goat
L-BSE
Oral transmission
PrPsc
Prion
RT-QuIC
Ultrasensitive detection
Journal
BMC research notes
ISSN: 1756-0500
Titre abrégé: BMC Res Notes
Pays: England
ID NLM: 101462768
Informations de publication
Date de publication:
07 Dec 2021
07 Dec 2021
Historique:
received:
13
09
2021
accepted:
23
11
2021
entrez:
8
12
2021
pubmed:
9
12
2021
medline:
15
12
2021
Statut:
epublish
Résumé
The spread of bovine spongiform encephalopathy (BSE) agent to small ruminants is still a major issue in the surveillance of transmissible spongiform encephalopathies (TSEs). L-type bovine spongiform encephalopathy (L-BSE) is an atypical form of BSE with an unknown zoonotic potential that is transmissible to cattle and small ruminants. Our current knowledge of bovine atypical prion strains in sheep and goat relies only on experimental transmission studies by intracranial inoculation. To assess oral susceptibility of goats to L-BSE, we orally inoculated five goats with cattle L-BSE brain homogenates and investigated pathogenic prion protein (PrP Despite a prolonged observation period of 80 months, all these animals and the uninfected controls did not develop clinical signs referable to TSEs and tested negative by standard diagnostics. Otherwise, RT-QuIC analysis showed seeding activity in five out of five examined brain samples. PrP
Identifiants
pubmed: 34876215
doi: 10.1186/s13104-021-05859-3
pii: 10.1186/s13104-021-05859-3
pmc: PMC8650279
doi:
Substances chimiques
Prion Proteins
0
Prions
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
442Subventions
Organisme : Ministero della Salute
ID : IZSPLV04/17RC
Organisme : Ministero della Salute
ID : IZSPLV04/19RC
Organisme : Ministero della Salute
ID : RF-2013-02354884
Informations de copyright
© 2021. The Author(s).
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