Acetoacetate protects macrophages from lactic acidosis-induced mitochondrial dysfunction by metabolic reprograming.


Journal

Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555

Informations de publication

Date de publication:
08 12 2021
Historique:
received: 07 10 2019
accepted: 09 11 2021
entrez: 9 12 2021
pubmed: 10 12 2021
medline: 11 1 2022
Statut: epublish

Résumé

Lactic acidosis, the extracellular accumulation of lactate and protons, is a consequence of increased glycolysis triggered by insufficient oxygen supply to tissues. Macrophages are able to differentiate from monocytes under such acidotic conditions, and remain active in order to resolve the underlying injury. Here we show that, in lactic acidosis, human monocytes differentiating into macrophages are characterized by depolarized mitochondria, transient reduction of mitochondrial mass due to mitophagy, and a significant decrease in nutrient absorption. These metabolic changes, resembling pseudostarvation, result from the low extracellular pH rather than from the lactosis component, and render these cells dependent on autophagy for survival. Meanwhile, acetoacetate, a natural metabolite produced by the liver, is utilized by monocytes/macrophages as an alternative fuel to mitigate lactic acidosis-induced pseudostarvation, as evidenced by retained mitochondrial integrity and function, retained nutrient uptake, and survival without the need of autophagy. Our results thus show that acetoacetate may increase tissue tolerance to sustained lactic acidosis.

Identifiants

pubmed: 34880237
doi: 10.1038/s41467-021-27426-x
pii: 10.1038/s41467-021-27426-x
pmc: PMC8655019
doi:

Substances chimiques

Acetoacetates 0
Protective Agents 0
Lactic Acid 33X04XA5AT
acetoacetic acid 4ZI204Y1MC

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

7115

Informations de copyright

© 2021. The Author(s).

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Auteurs

Clément Adam (C)

Univ Angers, Université de Nantes, INSERM, CRCINA, LabEx IGO, SFR ICAT, F-49000, Angers, France.

Léa Paolini (L)

Univ Angers, Université de Nantes, INSERM, CRCINA, LabEx IGO, SFR ICAT, F-49000, Angers, France.

Naïg Gueguen (N)

Univ Angers, CHU d'Angers, INSERM, CNRS, MitoVasc, SFR ICAT, F-49000, Angers, France.
Département de Biochimie et Génétique, CHU d'Angers, Angers, France.

Guillaume Mabilleau (G)

GEROM, Université d'Angers, Angers, France.
Département de Pathologie Cellulaire et Tissulaire, CHU d'Angers, Angers, France.

Laurence Preisser (L)

Univ Angers, Université de Nantes, INSERM, CRCINA, LabEx IGO, SFR ICAT, F-49000, Angers, France.

Simon Blanchard (S)

Univ Angers, Université de Nantes, INSERM, CRCINA, LabEx IGO, SFR ICAT, F-49000, Angers, France.
Laboratoire d'Immunologie et Allergologie, CHU d'Angers, Angers, France.

Pascale Pignon (P)

Univ Angers, Université de Nantes, INSERM, CRCINA, LabEx IGO, SFR ICAT, F-49000, Angers, France.

Florence Manero (F)

Univ Angers, SFR ICAT, SCIAM, F-49000, Angers, France.

Morgane Le Mao (M)

Univ Angers, CHU d'Angers, INSERM, CNRS, MitoVasc, SFR ICAT, F-49000, Angers, France.

Alain Morel (A)

Univ Angers, Université de Nantes, INSERM, CRCINA, LabEx IGO, SFR ICAT, F-49000, Angers, France.
Institut de Cancérologie de l'Ouest, F-49000, Angers, France.

Pascal Reynier (P)

Laboratoire de Biochimie et biologie moléculaire, CHU d'Angers, Angers, France.

Céline Beauvillain (C)

Univ Angers, Université de Nantes, INSERM, CRCINA, LabEx IGO, SFR ICAT, F-49000, Angers, France.
Laboratoire d'Immunologie et Allergologie, CHU d'Angers, Angers, France.

Yves Delneste (Y)

Univ Angers, Université de Nantes, INSERM, CRCINA, LabEx IGO, SFR ICAT, F-49000, Angers, France.
Laboratoire d'Immunologie et Allergologie, CHU d'Angers, Angers, France.

Vincent Procaccio (V)

Univ Angers, CHU d'Angers, INSERM, CNRS, MitoVasc, SFR ICAT, F-49000, Angers, France.
Département de Biochimie et Génétique, CHU d'Angers, Angers, France.

Pascale Jeannin (P)

Univ Angers, Université de Nantes, INSERM, CRCINA, LabEx IGO, SFR ICAT, F-49000, Angers, France. pascale.jeannin@univ-angers.fr.
Laboratoire d'Immunologie et Allergologie, CHU d'Angers, Angers, France. pascale.jeannin@univ-angers.fr.

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