Dimeric and high-resolution structures of Chlamydomonas Photosystem I from a temperature-sensitive Photosystem II mutant.
Journal
Communications biology
ISSN: 2399-3642
Titre abrégé: Commun Biol
Pays: England
ID NLM: 101719179
Informations de publication
Date de publication:
09 12 2021
09 12 2021
Historique:
received:
10
05
2021
accepted:
19
11
2021
entrez:
10
12
2021
pubmed:
11
12
2021
medline:
11
1
2022
Statut:
epublish
Résumé
Water molecules play a pivotal functional role in photosynthesis, primarily as the substrate for Photosystem II (PSII). However, their importance and contribution to Photosystem I (PSI) activity remains obscure. Using a high-resolution cryogenic electron microscopy (cryo-EM) PSI structure from a Chlamydomonas reinhardtii temperature-sensitive photoautotrophic PSII mutant (TSP4), a conserved network of water molecules - dating back to cyanobacteria - was uncovered, mainly in the vicinity of the electron transport chain (ETC). The high-resolution structure illustrated that the water molecules served as a ligand in every chlorophyll that was missing a fifth magnesium coordination in the PSI core and in the light-harvesting complexes (LHC). The asymmetric distribution of the water molecules near the ETC branches modulated their electrostatic landscape, distinctly in the space between the quinones and FX. The data also disclosed the first observation of eukaryotic PSI oligomerisation through a low-resolution PSI dimer that was comprised of PSI-10LHC and PSI-8LHC.
Identifiants
pubmed: 34887518
doi: 10.1038/s42003-021-02911-7
pii: 10.1038/s42003-021-02911-7
pmc: PMC8660910
doi:
Substances chimiques
Photosystem I Protein Complex
0
Photosystem II Protein Complex
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
1380Subventions
Organisme : NIGMS NIH HHS
ID : P41 GM103311
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM129325
Pays : United States
Informations de copyright
© 2021. The Author(s).
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