Nectar Yeast Community of Tropical Flowering Plants and Assessment of Their Osmotolerance and Xylitol-Producing Potential.
Journal
Current microbiology
ISSN: 1432-0991
Titre abrégé: Curr Microbiol
Pays: United States
ID NLM: 7808448
Informations de publication
Date de publication:
14 Dec 2021
14 Dec 2021
Historique:
received:
01
03
2021
accepted:
03
11
2021
entrez:
14
12
2021
pubmed:
15
12
2021
medline:
17
12
2021
Statut:
epublish
Résumé
Floral nectar is colonised by microbes, especially yeasts which alter the scent, temperature, and chemical composition of nectar, thereby playing an essential role in pollination. The yeast communities inhabiting the nectar of tropical flowers of India are not well explored. We isolated 48 yeast strains from seven different tropical flowering plants. Post MSP-PCR-based screening, 23 yeast isolates and two yeast-like fungi were identified, which belonged to 16 species of 12 genera viz. Candida (2 species), Aureobasidium (2 species), Metschnikowia (2 species), Meyerozyma (1 species), Saitozyma (1 species), Wickerhamomyces (1 species), Kodamaea (2 species), Pseudozyma (1 species), Starmerella (1 species), Hanseniaspora (1 species), Rhodosporidiobolus (1 species), Moesziomyces (1 species), and two putative novel species. All yeast strains were assessed for their osmotolerance abilities in high salt and sugar concentration. Among all the isolates, C. nivariensis (SRA2.2, SRA1.1 and SRA2.1), M. caribbica (SRA4.8 and SRA4.6), S. flava SRA4.2, and M. reukaufii SRA3.2 showed significant growth in high concentrations of sugar (40-50% glucose), as well as salt (12-15% NaCl). All 25 strains were also screened for their ability to utilise xylose to produce xylitol. Meyerozyma caribbica was the most efficient xylitol producer, wherein three strains of this species (SRA4.6, SRA4.1, and SRA4.8) generated 18.61 to 21.56 g l
Identifiants
pubmed: 34905093
doi: 10.1007/s00284-021-02700-9
pii: 10.1007/s00284-021-02700-9
doi:
Substances chimiques
Plant Nectar
0
Xylitol
VCQ006KQ1E
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
28Informations de copyright
© 2021. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
Références
Klaps J, Lievens B, Álvarez-Pérez S (2020) Towards a better understanding of the role of nectar-inhabiting yeasts in plant-animal interactions. Fungal Biol Biotechnol 7:1. https://doi.org/10.1186/s40694-019-0091-8
doi: 10.1186/s40694-019-0091-8
pubmed: 31921433
pmcid: 6947986
Sobhy IS, Baets D, Goelen T et al (2018) Sweet scents: nectar specialist yeasts enhance nectar attraction of a generalist aphid parasitoid without affecting survival. Front Plant Sci 9:1009. https://doi.org/10.3389/fpls.2018.01009
doi: 10.3389/fpls.2018.01009
pubmed: 30061909
pmcid: 6055026
Herrera CM, Pozo MI (2010) Nectar yeasts warm the flowers of a winter-blooming plant. Proc R Soc B Biol Sci 277:1827–1834. https://doi.org/10.1098/rspb.2009.2252
doi: 10.1098/rspb.2009.2252
Nicolson SW, de Veer L, Köhler A, Pirk CWW (2013) Honeybees prefer warmer nectar and less viscous nectar, regardless of sugar concentration. Proc R Soc B Biol Sci 280:20131597. https://doi.org/10.1098/rspb.2013.1597
doi: 10.1098/rspb.2013.1597
Vannette RL, Fukami T (2016) Nectar microbes can reduce secondary metabolites in nectar and alter effects on nectar consumption by pollinators. Ecology 97:1410–1419. https://doi.org/10.1890/15-0858.1
doi: 10.1890/15-0858.1
pubmed: 27459772
Golonka AM, Johnson BO, Freeman J, Hinson DW (2014) Impact of nectarivorous yeasts on Silene caroliniana’s scent. East Biol 3:1–26
Pozo MI, de Vega C, Canto A, Herrera CM (2009) Presence of yeasts in floral nectar is consistent with the hypothesis of microbial-mediated signaling in plant-pollinator interactions. Plant Signal Behav 4:1102–1104. https://doi.org/10.4161/psb.4.11.9874
doi: 10.4161/psb.4.11.9874
pubmed: 20009562
pmcid: 2819527
Rering CC, Beck JJ, Hall GW et al (2018) Nectar-inhabiting microorganisms influence nectar volatile composition and attractiveness to a generalist pollinator. New Phytol 220:750–759. https://doi.org/10.1111/nph.14809
doi: 10.1111/nph.14809
pubmed: 28960308
Dhami MK, Hartwig T, Fukami T (2016) Genetic basis of priority effects: insights from nectar yeast. Proc R Soc B Biol Sci 282:20161455. https://doi.org/10.1098/rspb.2016.1455
doi: 10.1098/rspb.2016.1455
Canto A, Herrera CM, Rodriguez R (2017) Nectar-living yeasts of a tropical host plant community: diversity and effects on community-wide floral nectar traits. PeerJ 5:e3517. https://doi.org/10.7717/peerj.3517
doi: 10.7717/peerj.3517
pubmed: 28717591
pmcid: 5511698
Akšić MF, Tosti T, Nedić N et al (2015) Influence of frost damage on the sugars and sugar alcohol composition in quince (Cydonia oblonga Mill.) floral nectar. Acta Physiol Plant. 37:1701. https://doi.org/10.1007/s11738-014-1701-y
doi: 10.1007/s11738-014-1701-y
Tokuoka K, Ishitani T, Chung WC (1992) Accumulation of polyols and sugars in some sugar-tolerant yeasts. J Gen Appl Microbiol 38:35–46. https://doi.org/10.2323/jgam.38.35
doi: 10.2323/jgam.38.35
Groleau D, Chevalier P, Yuen TLSTH (1995) Production of polyols and ethanol by the osmophilic yeast Zygosaccharomyces rouxii. Biotechnol Lett 17:315–320. https://doi.org/10.1007/BF01190645
doi: 10.1007/BF01190645
Tiwari S, Baghela A (2020) Challenges and prospects of xylitol production by conventional and non-conventional yeasts. In: Singh J, Gehlot P (eds) New and future developments in microbial biotechnology and bioengineering. Elsevier, pp 211–222
da Silveira FA, Fernandes TAR, Bragança CRS et al (2020) Isolation of xylose-assimilating yeasts and optimisation of xylitol production by a new Meyerozyma guilliermondii strain. Int Microbiol 23:325–334. https://doi.org/10.1007/s10123-019-00105-0
doi: 10.1007/s10123-019-00105-0
pubmed: 31813072
Misra S, Raghuwanshi S, Gupta P et al (2012) Fermentation behavior of osmophilic yeast Candida tropicalis isolated from the nectar of Hibiscus rosa sinensis flowers for xylitol production. Antonie Van Leeuwenhoek 101:393–402. https://doi.org/10.1007/s10482-011-9646-2
doi: 10.1007/s10482-011-9646-2
pubmed: 21956659
Carneiro CVGC, E Silva FC de P, Almeida JRM (2019) Xylitol production: Identification and comparison of new producing yeasts. Microorganisms 7:484. https://doi.org/10.3390/microorganisms7110484
Aamir S (2015) A rapid and efficient method of fungal genomic DNA extraction, suitable for PCR based molecular methods. Plant Pathol Quar 5:74–81. https://doi.org/10.5943/ppq/5/2/6
doi: 10.5943/ppq/5/2/6
Ramírez-Castrillón M, Mendes SDC, Inostroza-Ponta M, Valente P (2014) (GTG)5 MSP-PCR fingerprinting as a technique for discrimination of wine associated yeasts? PLoS One 9:e105870. https://doi.org/10.1371/journal.pone.010587020
doi: 10.1371/journal.pone.010587020
pubmed: 25171185
pmcid: 4149466
Kurtzman CP, Robnett CJ (1998) Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie Van Leeuwenhoek 73:331–371. https://doi.org/10.1023/A:1001761008817
doi: 10.1023/A:1001761008817
pubmed: 9850420
Nguyen LT, Schmidt HA, von Haeseler A, Minh BQ (2015) IQ-TREE: a fast and effective stochastic algorithm for estimating maximum-likelihood phylogenies. Mol Biol Evol. https://doi.org/10.1093/molbev/msu300
doi: 10.1093/molbev/msu300
pubmed: 26659249
pmcid: 4760084
Ok T, Hashinaga F (1997) Identification of sugar-tolerant yeasts isolated from high-sugar fermented vegetable extracts. J Gen Appl Microbiol 43:39–47. https://doi.org/10.2323/jgam.43.39
doi: 10.2323/jgam.43.39
pubmed: 12501352
Osho A (2005) Ethanol and sugar tolerance of wine yeasts isolated from fermenting cashew apple juice. African J Biotechnol 4:660–662. https://doi.org/10.5897/AJB2005.000-3119
doi: 10.5897/AJB2005.000-3119
Tiwari S, Avchar R, Arora R et al (2020) Xylanolytic and ethanologenic potential of gut associated yeasts from different species of termites from India. Mycobiology 48:501–511. https://doi.org/10.1080/12298093.2020.1830742
doi: 10.1080/12298093.2020.1830742
pubmed: 33312017
pmcid: 7717550
Vu D, Groenewald M, Szöke S et al (2016) DNA barcoding analysis of more than 9 000 yeast isolates contributes to quantitative thresholds for yeast species and genera delimitation. Stud Mycol 85:91–105. https://doi.org/10.1016/j.simyco.2016.11.007
doi: 10.1016/j.simyco.2016.11.007
pubmed: 28050055
pmcid: 5192050
Belisle M, Peay KG, Fukami T (2012) Flowers as Islands: Spatial Distribution of Nectar-Inhabiting Microfungi among Plants of Mimulus aurantiacus, a Hummingbird-Pollinated Shrub. Microb Ecol 63:711–718. https://doi.org/10.1007/s00248-011-9975-8
doi: 10.1007/s00248-011-9975-8
pubmed: 22080257
Canto A, Herrera CM (2012) Micro-organisms behind the pollination scenes: Microbial imprint on floral nectar sugar variation in a tropical plant community. Ann Botany 110:1173–1183. https://doi.org/10.1093/aob/mcs183
doi: 10.1093/aob/mcs183
de Vega C, Guzmán B, Lachance MA et al (2012) Metschnikowia proteae sp. nov., a nectarivorous insect-associated yeast species from Africa. Int J Syst Evol Microbiol 62:2538–2545. https://doi.org/10.1099/ijs.0.040790-0
doi: 10.1099/ijs.0.040790-0
pubmed: 22407789
Mittelbach M, Yurkov AM, Nocentini D et al (2015) Nectar sugars and bird visitation define a floral niche for basidiomycetous yeast on the Canary Islands. BMC Ecol 15:2. https://doi.org/10.1186/s12898-015-0036-x
doi: 10.1186/s12898-015-0036-x
pubmed: 25638173
pmcid: 4318194
Graham JR, Ellis JD, Benda ND et al (2011) Kodamaea ohmeri (Ascomycota: Saccharomycotina presence in commercial Bombus impatiens Cresson and feral Bombus pensylvanicus DeGeer (Hymenoptera: Apidae) colonies. J Apic Res 50:218–226. https://doi.org/10.3896/IBRA.1.50.3.06
doi: 10.3896/IBRA.1.50.3.06
Lachance MA, Kurtzman CP (2011) Kodamaea Y: Yamada, T. Suzuki, Matsuda & Mikata emend. Rosa, Lachance, Starmer, Barker, Bowles & Schlag-Edler (1999). In: The Yeasts. Elsevier, pp 483–490
Morris MM, Frixione NJ, Burkert AC et al (2019) Microbial abundance, composition, and function in nectar are shaped by flower visitor identity. FEMS Microbiol Ecol 96:fiaa003. https://doi.org/10.1093/femsec/fiaa003
doi: 10.1093/femsec/fiaa003
Herrera CM, de Vega C, Canto A, Pozo MI (2009) Yeasts in floral nectar: a quantitative survey. Ann Bot 103:1415–1423
doi: 10.1093/aob/mcp026
Brysch-Herzberg M (2004) Ecology of yeasts in plant-bumblebee mutualism in Central Europe. FEMS Microbiol Ecol 50:87–100. https://doi.org/10.1016/j.femsec.2004.06.003
doi: 10.1016/j.femsec.2004.06.003
pubmed: 19712367
Struyf N, van der Maelen E, Hemdane S et al (2017) Bread dough and baker’s yeast: an uplifting synergy. Compr Rev Food Sci Food Saf 16:850–867. https://doi.org/10.1111/1541-4337.12282
doi: 10.1111/1541-4337.12282
pubmed: 33371607
Zhou N, Schifferdecker AJ, Gamero A et al (2017) Kazachstania gamospora and Wickerhamomyces subpelliculosus: Two alternative baker’s yeasts in the modern bakery. Int J Food Microbiol 250:45–58. https://doi.org/10.1016/j.ijfoodmicro.2017.03.013
doi: 10.1016/j.ijfoodmicro.2017.03.013
pubmed: 28365494
Atzmüller D, Ullmann N, Zwirzitz A (2020) Identification of genes involved in xylose metabolism of Meyerozyma guilliermondii and their genetic engineering for increased xylitol production. AMB Express. https://doi.org/10.1186/s13568-020-01012-8
doi: 10.1186/s13568-020-01012-8
pubmed: 32314068
pmcid: 7171046
Saputra H, Thontowi A, Kholida LN, Kanti A (2020) Efficiency of xylitol production from Meyerozyma caribbica Y67 with cell initiation and volume fermentation. In: IOP conference series: earth and environmental science. 439:012032. https://doi.org/10.1088/1755-1315/439/1/012032