Electrochemical immunosensor for detection of avian Salmonellosis based on electroactive reduced graphene oxide (rGO) modified electrode.

Biosensing Techniques
Antibody Antigen Electrochemical Fluorine doped tin oxide Graphene oxide Salmonellosis

Journal

Bioelectrochemistry (Amsterdam, Netherlands)
ISSN: 1878-562X
Titre abrégé: Bioelectrochemistry
Pays: Netherlands
ID NLM: 100953583

Informations de publication

Date de publication:
Apr 2022
Historique:
received: 01 11 2021
revised: 26 11 2021
accepted: 06 12 2021
pubmed: 16 12 2021
medline: 3 3 2022
entrez: 15 12 2021
Statut: ppublish

Résumé

A novel reduced graphene oxide based (rGO) fluorine doped tin oxide (FTO) electrode was fabricated to explore the interaction of Salmonella serovars (Salmonella gallinarum, and Salmonella pullorum) with specific antibodies. Reduced graphene oxide (rGO) was labelled with S. gal and S. pul-Ab via carbodiimide activation. The biophysical characterization of fabricated electrode was done by Fourier-transform infrared spectroscopy (FT-IR), Raman Spectroscopy, X-ray Diffraction (XRD), Scanning Electron Microscopy (SEM), Energy Dispersive X-Ray Analysis (EDX), cyclic voltammetry (CV), and differential pulse voltammetry (DPV), and electrochemical impedance spectroscopy (EIS). The optimization of fabricated electrode was done for various physico-chemical parameters. Under optimum conditions, the immunosensor exhibited a linear detection range (1- 1 × 10

Identifiants

DOI: 10.1016/j.bioelechem.2021.108036 PMID: 34906818
pubmed: 34906818
pii: S1567-5394(21)00299-1
doi: 10.1016/j.bioelechem.2021.108036
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

108036

Informations de copyright

Copyright © 2021 Elsevier B.V. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Auteurs

Subhasis Mahari (S)

DBT - National Institute of Animal Biotechnology (NIAB), Hyderabad 500032, Telangana, India; DBT-Regional Centre for Biotechnology (RCB), Faridabad 121001, Haryana, India.

Sonu Gandhi (S)

DBT - National Institute of Animal Biotechnology (NIAB), Hyderabad 500032, Telangana, India; DBT-Regional Centre for Biotechnology (RCB), Faridabad 121001, Haryana, India. Electronic address: gandhi@niab.org.in.

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Classifications MeSH

questionsmedicales.fr › Techniques d'investigation › Techniques de sonde moléculaire › Techniques de biocapteur › Electrochemical immunosensor for detection of...