Ex Vivo Culture of Human Cranial Suture Cells.
Cell culture
Craniosynostosis
Osteoblast
Stem cell
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2022
2022
Historique:
entrez:
16
12
2021
pubmed:
17
12
2021
medline:
20
1
2022
Statut:
ppublish
Résumé
The culture of human cranial suture cells, including their osteoblasts, is an important asset to developmental and molecular biologists to allow study the molecular biology ex vivo. The use of cell cultures by bone biologists to investigate pathological bone formation has been well established (Marie et al. Vitro Cell Dev Biol 25:373-380, 1989), and the use of cell culture techniques was subsequently applied to investigate craniosynostosis (Marie. J Bone Miner Res 9(12):1847-1850, 1994). Cell cultures from fused, fusing, and fused cranial sutures allow comparative studies of cellular behavior from sutures with pathological craniosynostosis and those with unaffected sutures (Coussens et al. J Cell Physiol 218(1):183-191, 2009; Coussens et al. Differentiation 76(5):531-545, 2008).In addition to using this limited human resource for primary investigations, these human cell studies may be targeted to complement and help verify the findings of investigative studies undertaken using the more readily accessible animal cranial sutures. It is important, however, to remember that there may be critical differences in the animal genome which could impact on cellular function.This chapter describes the techniques for human suture cell culture and storage which have been used successfully since 2005 in the craniosynostosis laboratories in Adelaide.
Identifiants
pubmed: 34913125
doi: 10.1007/978-1-0716-1847-9_14
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
215-222Informations de copyright
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
Références
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