A Genome-Wide CRISPR/Cas9-Based Screen Identifies Heparan Sulfate Proteoglycans as Ligands of Killer-Cell Immunoglobulin-Like Receptors.
CRISPR
KIR
NK cells
heparan sulfate
screen
Journal
Frontiers in immunology
ISSN: 1664-3224
Titre abrégé: Front Immunol
Pays: Switzerland
ID NLM: 101560960
Informations de publication
Date de publication:
2021
2021
Historique:
received:
19
10
2021
accepted:
11
11
2021
entrez:
17
12
2021
pubmed:
18
12
2021
medline:
1
3
2022
Statut:
epublish
Résumé
While human leukocyte antigen (HLA) and HLA-like proteins comprise an overwhelming majority of known ligands for NK-cell receptors, the interactions of NK-cell receptors with non-conventional ligands, particularly carbohydrate antigens, is less well described. We previously found through a bead-based HLA screen that KIR3DS1, a formerly orphan member of the killer-cell immunoglobulin-like receptor (KIR) family, binds to HLA-F. In this study, we assessed the ligand binding profile of KIR3DS1 to cell lines using Fc fusion constructs, and discovered that KIR3DS1-Fc exhibited binding to several human cell lines including ones devoid of HLA. To identify these non-HLA ligands, we developed a magnetic enrichment-based genome-wide CRISPR/Cas9 knock-out screen approach, and identified enzymes involved in the biosynthesis of heparan sulfate as crucial for the binding of KIR3DS1-Fc to K562 cells. This interaction between KIR3DS1 and heparan sulfate was confirmed
Identifiants
pubmed: 34917099
doi: 10.3389/fimmu.2021.798235
pmc: PMC8669139
doi:
Substances chimiques
Heparan Sulfate Proteoglycans
0
KIR3DS1 protein, human
0
Ligands
0
Receptors, KIR
0
Receptors, KIR3DS1
0
CRISPR-Associated Protein 9
EC 3.1.-
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
798235Subventions
Organisme : NIAID NIH HHS
ID : R01 AI067031
Pays : United States
Organisme : NIAID NIH HHS
ID : P01 AI104715
Pays : United States
Organisme : NIAID NIH HHS
ID : F31 AI116366
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM007753
Pays : United States
Informations de copyright
Copyright © 2021 Klein, Hölzemer, Wang, Kim, Dugan, Jost, Altfeld and Garcia-Beltran.
Déclaration de conflit d'intérêts
Author HD is employed by Adimab, LLC. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Références
Oncogene. 2007 Mar 1;26(10):1407-16
pubmed: 16953225
Front Immunol. 2012 Jan 03;2:88
pubmed: 22566877
Oncogene. 2003 Jan 16;22(2):274-80
pubmed: 12527896
J Intern Med. 2016 Jul;280(1):97-113
pubmed: 26749537
Biomed Res Int. 2015;2015:549417
pubmed: 26236728
Front Immunol. 2012 Sep 17;3:289
pubmed: 23060877
Mol Cell Proteomics. 2019 Nov;18(11):2310-2323
pubmed: 31308249
Nat Immunol. 2016 Sep;17(9):1067-74
pubmed: 27455421
Cell. 1991 Feb 22;64(4):841-8
pubmed: 1847668
Dev Cell. 2006 May;10(5):625-34
pubmed: 16678777
Biochem J. 1995 Sep 1;310 ( Pt 2):497-505
pubmed: 7654188
J Immunol. 2011 Jul 1;187(1):11-9
pubmed: 21690332
J Immunol. 2013 Nov 15;191(10):5256-67
pubmed: 24127555
Nature. 2011 Oct 23;479(7373):401-5
pubmed: 22020283
Cell. 2011 Mar 4;144(5):646-74
pubmed: 21376230
Nucleic Acids Res. 2015 Jan;43(Database issue):D423-31
pubmed: 25414341
Nucleic Acids Res. 2013 Jan;41(Database issue):D1234-40
pubmed: 23180793
Cancer Immunol Res. 2021 Feb;9(2):156-169
pubmed: 33229411
Clin Dev Immunol. 2013;2013:427696
pubmed: 24066005
Science. 2015 Nov 27;350(6264):1096-101
pubmed: 26472758
Nat Rev Immunol. 2006 Sep;6(9):633-43
pubmed: 16917509
Front Immunol. 2013 Mar 20;4:69
pubmed: 23518691
J Biol Chem. 2005 May 27;280(21):20516-23
pubmed: 15778504
Curr Opin Chem Biol. 2000 Dec;4(6):626-31
pubmed: 11102866
Nat Immunol. 2005 Sep;6(9):861-2
pubmed: 16116461
PLoS One. 2016 Sep 20;11(9):e0163297
pubmed: 27649529
Blood. 2010 Nov 11;116(19):3865-74
pubmed: 20733159
J Clin Invest. 2001 Jan;107(2):R9-R14
pubmed: 11160142
Cold Spring Harb Perspect Biol. 2011 Jul 01;3(7):
pubmed: 21690215
J Clin Invest. 2017 Jun 30;127(7):2777-2788
pubmed: 28581441
Angew Chem Int Ed Engl. 2002 Feb 1;41(3):391-412
pubmed: 12491369
Front Immunol. 2013 Dec 09;4:422
pubmed: 24367364
FASEB J. 2010 Feb;24(2):436-50
pubmed: 19812376
J Biol Chem. 1999 Dec 17;274(51):36267-73
pubmed: 10593915
J Immunol. 2000 Jan 1;164(1):319-28
pubmed: 10605026
Proc Natl Acad Sci U S A. 2002 Feb 5;99(3):1229-34
pubmed: 11830659
J Proteome Res. 2009 Feb;8(2):712-20
pubmed: 19196184