Combinatorial use of environmental stresses and genetic engineering to increase ethanol titres in cyanobacteria.
Carbon partitioning
Cyanobacteria
Environmental stress
Ethanol
Microbial pathway engineering
Synechocystis PCC 6803
Synthetic biology
Journal
Biotechnology for biofuels
ISSN: 1754-6834
Titre abrégé: Biotechnol Biofuels
Pays: England
ID NLM: 101316935
Informations de publication
Date de publication:
17 Dec 2021
17 Dec 2021
Historique:
received:
04
10
2021
accepted:
05
12
2021
entrez:
18
12
2021
pubmed:
19
12
2021
medline:
19
12
2021
Statut:
epublish
Résumé
Current industrial bioethanol production by yeast through fermentation generates carbon dioxide. Carbon neutral bioethanol production by cyanobacteria uses biological fixation (photosynthesis) of carbon dioxide or other waste inorganic carbon sources, whilst being sustainable and renewable. The first ethanologenic cyanobacterial process was developed over two decades ago using Synechococcus elongatus PCC 7942, by incorporating the recombinant pdc and adh genes from Zymomonas mobilis. Further engineering has increased bioethanol titres 24-fold, yet current levels are far below what is required for industrial application. At the heart of the problem is that the rate of carbon fixation cannot be drastically accelerated and carbon partitioning towards bioethanol production impacts on cell fitness. Key progress has been achieved by increasing the precursor pyruvate levels intracellularly, upregulating synthetic genes and knocking out pathways competing for pyruvate. Studies have shown that cyanobacteria accumulate high proportions of carbon reserves that are mobilised under specific environmental stresses or through pathway engineering to increase ethanol production. When used in conjunction with specific genetic knockouts, they supply significantly more carbon for ethanol production. This review will discuss the progress in generating ethanologenic cyanobacteria through chassis engineering, and exploring the impact of environmental stresses on increasing carbon flux towards ethanol production.
Identifiants
pubmed: 34920731
doi: 10.1186/s13068-021-02091-w
pii: 10.1186/s13068-021-02091-w
pmc: PMC8684110
doi:
Types de publication
Journal Article
Review
Langues
eng
Pagination
240Subventions
Organisme : Engineering and Physical Sciences Research Council
ID : EP/S01778X/1
Organisme : Office of Naval Research Global
ID : N62909-18-1-2137
Informations de copyright
© 2021. The Author(s).
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