Deciphering the physiological response of Escherichia coli under high ATP demand.
ATP homeostasis
central metabolism
glycolysis
kinetic model
metabolic engineering
Journal
Molecular systems biology
ISSN: 1744-4292
Titre abrégé: Mol Syst Biol
Pays: England
ID NLM: 101235389
Informations de publication
Date de publication:
12 2021
12 2021
Historique:
revised:
30
11
2021
received:
15
06
2021
accepted:
30
11
2021
entrez:
20
12
2021
pubmed:
21
12
2021
medline:
27
1
2022
Statut:
ppublish
Résumé
One long-standing question in microbiology is how microbes buffer perturbations in energy metabolism. In this study, we systematically analyzed the impact of different levels of ATP demand in Escherichia coli under various conditions (aerobic and anaerobic, with and without cell growth). One key finding is that, under all conditions tested, the glucose uptake increases with rising ATP demand, but only to a critical level beyond which it drops markedly, even below wild-type levels. Focusing on anaerobic growth and using metabolomics and proteomics data in combination with a kinetic model, we show that this biphasic behavior is induced by the dual dependency of the phosphofructokinase on ATP (substrate) and ADP (allosteric activator). This mechanism buffers increased ATP demands by a higher glycolytic flux but, as shown herein, it collapses under very low ATP concentrations. Model analysis also revealed two major rate-controlling steps in the glycolysis under high ATP demand, which could be confirmed experimentally. Our results provide new insights on fundamental mechanisms of bacterial energy metabolism and guide the rational engineering of highly productive cell factories.
Identifiants
pubmed: 34928538
doi: 10.15252/msb.202110504
pmc: PMC8686765
doi:
Substances chimiques
Adenosine Triphosphate
8L70Q75FXE
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e10504Informations de copyright
© 2021 The Authors Published under the terms of the CC BY 4.0 license.
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