Investigation of sugar binding kinetics of the E. coli sugar/H
MFS
SSM
XylE
bioenergetics
energy coupling
induced fit
membrane transport
sugar transporter
Journal
The Journal of biological chemistry
ISSN: 1083-351X
Titre abrégé: J Biol Chem
Pays: United States
ID NLM: 2985121R
Informations de publication
Date de publication:
02 2022
02 2022
Historique:
received:
15
09
2021
revised:
11
12
2021
accepted:
13
12
2021
pubmed:
21
12
2021
medline:
29
4
2022
entrez:
20
12
2021
Statut:
ppublish
Résumé
Bacterial transporters are difficult to study using conventional electrophysiology because of their low transport rates and the small size of bacterial cells. Here, we applied solid-supported membrane-based electrophysiology to derive kinetic parameters of sugar translocation by the Escherichia coli xylose permease (XylE), including functionally relevant mutants. Many aspects of the fucose permease (FucP) and lactose permease (LacY) have also been investigated, which allow for more comprehensive conclusions regarding the mechanism of sugar translocation by transporters of the major facilitator superfamily. In all three of these symporters, we observed sugar binding and transport in real time to determine K
Identifiants
pubmed: 34929170
pii: S0021-9258(21)01315-6
doi: 10.1016/j.jbc.2021.101505
pmc: PMC8784342
pii:
doi:
Substances chimiques
Escherichia coli Proteins
0
LacY protein, E coli
0
Membrane Transport Proteins
0
Monosaccharide Transport Proteins
0
Sugars
0
Symporters
0
Glucose
IY9XDZ35W2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
101505Informations de copyright
Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Conflict of interest The authors declare that they have no conflicts of interest with the contents of this article.