Identification of components in Kazakhstan honeys that correlate with antimicrobial activity against wound and skin infecting microorganisms.
Acinetobacter baumannii
/ drug effects
Anti-Infective Agents
/ chemistry
Enterococcus faecalis
/ drug effects
Escherichia coli
/ drug effects
Honey
/ analysis
Humans
Kazakhstan
Malassezia
/ drug effects
Methicillin-Resistant Staphylococcus aureus
/ drug effects
Microbial Sensitivity Tests
Phytochemicals
/ chemistry
Polyphenols
/ chemistry
Pseudomonas aeruginosa
/ drug effects
Skin Diseases, Bacterial
/ microbiology
Wound Infection
/ microbiology
Antimicrobial activity
Kazakhstan honeys
Liquid chromatography with mass spectrometry
Polyphenols
Skin disease
Wound infections
Journal
BMC complementary medicine and therapies
ISSN: 2662-7671
Titre abrégé: BMC Complement Med Ther
Pays: England
ID NLM: 101761232
Informations de publication
Date de publication:
20 Dec 2021
20 Dec 2021
Historique:
received:
21
09
2020
accepted:
17
11
2021
entrez:
21
12
2021
pubmed:
22
12
2021
medline:
7
1
2022
Statut:
epublish
Résumé
Antimicrobial drug resistance is a major public health threat that can render infections including wound and skin infections untreatable. The discovery of new antimicrobials is critical. Approaches to discover novel antimicrobial therapies have included investigating the antimicrobial activity of natural sources such as honey. In this study, the anti-microbial activity and chemical composition of 12 honeys from Kazakhstan and medical grade manuka honey were investigated. Agar well diffusion and broth culture assays were used to determine anti-microbial activity against a range of skin and wound infecting micro-organisms. Folin-Ciocalteu method was used to determine the total phenol content of the honeys and non-targeted liquid chromatography analysis was performed to identify components that correlated with antimicrobial activity. In the well diffusion assay, the most susceptible micro-organisms were a clinical isolate of Methicillin resistant Staphylococcus aureus (MRSA) and Enterococcus faecalis (ATCC 19433). Buckwheat & multi-floral honey from Kazakhstan demonstrated the highest antimicrobial activity against these two micro-organisms. Kazakhstan honeys with a buckwheat floral source, and manuka honey had the highest total phenol content. Non-targeted liquid chromatography analysis identified components that correlated with anti-microbial activity as hydroxyphenyl acetic acid, p-coumaric acid, (1H)-quinolinone, and abscisic acid. The Kazakhstan honeys selected in this study demonstrated antimicrobial activity against wound and skin infecting micro-organisms. Compounds identified as correlating with antimicrobial activity could be considered as potential bioactive agents for the treatment of wound and skin infections.
Sections du résumé
BACKGROUND
BACKGROUND
Antimicrobial drug resistance is a major public health threat that can render infections including wound and skin infections untreatable. The discovery of new antimicrobials is critical. Approaches to discover novel antimicrobial therapies have included investigating the antimicrobial activity of natural sources such as honey. In this study, the anti-microbial activity and chemical composition of 12 honeys from Kazakhstan and medical grade manuka honey were investigated.
METHODS
METHODS
Agar well diffusion and broth culture assays were used to determine anti-microbial activity against a range of skin and wound infecting micro-organisms. Folin-Ciocalteu method was used to determine the total phenol content of the honeys and non-targeted liquid chromatography analysis was performed to identify components that correlated with antimicrobial activity.
RESULTS
RESULTS
In the well diffusion assay, the most susceptible micro-organisms were a clinical isolate of Methicillin resistant Staphylococcus aureus (MRSA) and Enterococcus faecalis (ATCC 19433). Buckwheat & multi-floral honey from Kazakhstan demonstrated the highest antimicrobial activity against these two micro-organisms. Kazakhstan honeys with a buckwheat floral source, and manuka honey had the highest total phenol content. Non-targeted liquid chromatography analysis identified components that correlated with anti-microbial activity as hydroxyphenyl acetic acid, p-coumaric acid, (1H)-quinolinone, and abscisic acid.
CONCLUSIONS
CONCLUSIONS
The Kazakhstan honeys selected in this study demonstrated antimicrobial activity against wound and skin infecting micro-organisms. Compounds identified as correlating with antimicrobial activity could be considered as potential bioactive agents for the treatment of wound and skin infections.
Identifiants
pubmed: 34930218
doi: 10.1186/s12906-021-03466-0
pii: 10.1186/s12906-021-03466-0
pmc: PMC8690519
doi:
Substances chimiques
Anti-Infective Agents
0
Phytochemicals
0
Polyphenols
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
300Subventions
Organisme : Ministry of Education and Science Republic of Kazakhstan
ID : АР05135293
Informations de copyright
© 2021. The Author(s).
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