Evaluation of four phosphopeptide enrichment strategies for mass spectrometry-based proteomic analysis.

Chromatography, Affinity / methods Mass Spectrometry Phosphopeptides / analysis Phosphorylation Proteomics / methods Titanium / chemistry
mass spectrometry phos-tag phosphopeptide enrichment proteomics titanium dioxide

Journal

Proteomics
ISSN: 1615-9861
Titre abrégé: Proteomics
Pays: Germany
ID NLM: 101092707

Informations de publication

Date de publication:
04 2022
Historique:
revised: 07 12 2021
received: 06 09 2021
accepted: 14 12 2021
pubmed: 22 12 2021
medline: 6 4 2022
entrez: 21 12 2021
Statut: ppublish

Résumé

Information about phosphorylation status can be used to prioritize and characterize biological processes in the cell. Various analytical strategies have been proposed to address the complexity of phosphorylation status and comprehensively identify phosphopeptides. In this study, we evaluated four strategies for phosphopeptide enrichment, using titanium dioxide (TiO

Identifiants

DOI: 10.1002/pmic.202100216 PMID: 34932266
pubmed: 34932266
doi: 10.1002/pmic.202100216
doi:

Substances chimiques

Phosphopeptides 0
Titanium D1JT611TNE

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

e2100216

Informations de copyright

© 2021 Wiley-VCH GmbH.

Références

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Auteurs

Yoko Ino (Y)

Advanced Medical Research Center, Yokohama City University, Yokohama, Japan.
Graduate School of Health Sciences, Gunma Paz University, Takasaki City, Gunma, Japan.

Eiji Kinoshita (E)

Department of Human Nutrition, Faculty of Human Sciences, Hiroshima Bunkyo University, Hiroshima, Japan.

Emiko Kinoshita-Kikuta (E)

Department of Functional Molecular Science, Graduate School of Biomedical & Health Sciences, Hiroshima University, Hiroshima, Japan.

Tomoko Akiyama (T)

Advanced Medical Research Center, Yokohama City University, Yokohama, Japan.

Yusuke Nakai (Y)

Advanced Medical Research Center, Yokohama City University, Yokohama, Japan.

Kohei Nishino (K)

Kuramoto Division, Technical Support Department, Tokushima University, Tokushima, Japan.

Makoto Osada (M)

Graduate School of Health Sciences, Gunma Paz University, Takasaki City, Gunma, Japan.

Akihide Ryo (A)

Advanced Medical Research Center, Yokohama City University, Yokohama, Japan.
Department of Microbiology, Yokohama City University School of Medicine, Yokohama, Japan.

Hisashi Hirano (H)

Advanced Medical Research Center, Yokohama City University, Yokohama, Japan.

Tohru Koike (T)

Department of Functional Molecular Science, Graduate School of Biomedical & Health Sciences, Hiroshima University, Hiroshima, Japan.

Yayoi Kimura (Y)

Advanced Medical Research Center, Yokohama City University, Yokohama, Japan.
ORCID: 0000-0002-4612-4602

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Classifications MeSH

questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Chromatographie › Chromatographie en phase liquide › Chromatographie d'affinité › Evaluation of four phosphopeptide enrichment...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Spectrométrie de masse › Evaluation of four phosphopeptide enrichment...
questionsmedicales.fr › Acides aminés, peptides et protéines › Peptides › Phosphopeptides › Evaluation of four phosphopeptide enrichment...
questionsmedicales.fr › Métabolisme › Phosphorylation › Evaluation of four phosphopeptide enrichment...
questionsmedicales.fr › Disciplines des sciences naturelles › Chimie › Biochimie › Protéomique › Evaluation of four phosphopeptide enrichment...
questionsmedicales.fr › Produits chimiques inorganiques › Métaux › Métaux légers › Titane › Evaluation of four phosphopeptide enrichment...