Comparison of Performance of Two Stool DNA Tests and a Fecal Immunochemical Test in Detecting Colorectal Neoplasm: A Multicenter Diagnostic Study.


Journal

Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology
ISSN: 1538-7755
Titre abrégé: Cancer Epidemiol Biomarkers Prev
Pays: United States
ID NLM: 9200608

Informations de publication

Date de publication:
01 Mar 2022
Historique:
received: 16 08 2021
revised: 25 10 2021
accepted: 13 12 2021
pubmed: 23 12 2021
medline: 3 5 2022
entrez: 22 12 2021
Statut: ppublish

Résumé

The most widely used noninvasive screening tests for colorectal cancer are fecal occult blood tests. Stool DNA test was developed in recent years. However, direct comparative analyses of these tests within the same population are still sparse. A total of 2,842 participants who visited outpatient clinics or cancer screening centers were enrolled. Stool DNA test-I (KRAS, BMP3, NDRG4, and hemoglobin immunochemical tests), stool DNA test-II (SDC2 and SFRP2 tests), and fecal immunochemical test (FIT) alone were performed and colonoscopy was used as the gold standard among 2,240 participants. Forty-two and 302 participants had colorectal cancer and advanced adenomas (AA), respectively. The sensitivity for colorectal cancer of stool DNA test-I, -II, and FIT was 90.5%, 92.9%, and 81.0%, respectively. The sensitivity for advanced neoplasm (AN; colorectal cancer plus AA) of stool DNA test-I, -II, and FIT was 34.9%, 42.2%, and 25.9%, respectively. The specificity of stool DNA test-I, -II, and FIT was 91.4%, 93.3%, and 96.8%, respectively, among those with negative results on colonoscopy. When the specificity of FIT was adjusted to match that of stool DNA tests by changing the threshold, no significant difference was seen in the sensitivities among the three tests for detecting colorectal cancer. For AN, the sensitivity of FIT was higher than DNA test-I and similar to DNA test-II under the same specificities. There was no significant advantage of the two stool DNA tests compared with FIT in detecting colorectal cancer or AN in this study. Our findings do not support extensive use of stool DNA tests instead of FIT.

Sections du résumé

BACKGROUND BACKGROUND
The most widely used noninvasive screening tests for colorectal cancer are fecal occult blood tests. Stool DNA test was developed in recent years. However, direct comparative analyses of these tests within the same population are still sparse.
METHODS METHODS
A total of 2,842 participants who visited outpatient clinics or cancer screening centers were enrolled. Stool DNA test-I (KRAS, BMP3, NDRG4, and hemoglobin immunochemical tests), stool DNA test-II (SDC2 and SFRP2 tests), and fecal immunochemical test (FIT) alone were performed and colonoscopy was used as the gold standard among 2,240 participants. Forty-two and 302 participants had colorectal cancer and advanced adenomas (AA), respectively.
RESULTS RESULTS
The sensitivity for colorectal cancer of stool DNA test-I, -II, and FIT was 90.5%, 92.9%, and 81.0%, respectively. The sensitivity for advanced neoplasm (AN; colorectal cancer plus AA) of stool DNA test-I, -II, and FIT was 34.9%, 42.2%, and 25.9%, respectively. The specificity of stool DNA test-I, -II, and FIT was 91.4%, 93.3%, and 96.8%, respectively, among those with negative results on colonoscopy. When the specificity of FIT was adjusted to match that of stool DNA tests by changing the threshold, no significant difference was seen in the sensitivities among the three tests for detecting colorectal cancer. For AN, the sensitivity of FIT was higher than DNA test-I and similar to DNA test-II under the same specificities.
CONCLUSIONS CONCLUSIONS
There was no significant advantage of the two stool DNA tests compared with FIT in detecting colorectal cancer or AN in this study.
IMPACT CONCLUSIONS
Our findings do not support extensive use of stool DNA tests instead of FIT.

Identifiants

pubmed: 34933958
pii: 1055-9965.EPI-21-0991
doi: 10.1158/1055-9965.EPI-21-0991
doi:

Substances chimiques

DNA, Neoplasm 0
DNA 9007-49-2

Types de publication

Journal Article Multicenter Study Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

654-661

Informations de copyright

©2021 American Association for Cancer Research.

Auteurs

Peng Jin (P)

Senior Department of Gastroenterology, the First Medical Center of Chinese PLA General Hospital, Beijing, China.
Department of Gastroenterology, the Seventh Medical Center of Chinese PLA General Hospital, Beijing, China.

Peng You (P)

Department of Gastroenterology, Peking University People's Hospital, Beijing, China.

Jingyuan Fang (J)

Division of Gastroenterology and Hepatology, Shanghai Jiao Tong University School of Medicine Affiliated Renji Hospital, Shanghai, China.

Qian Kang (Q)

Outpatient Department of Tai Hu, Eastern Medical District of Chinese PLA General Hospital, Beijing, China.

Fang Gu (F)

Department of Gastroenterology, Peking University Third Hospital, Beijing, China.

Yunlong Cai (Y)

Endoscopy Center, Peking University First Hospital, Beijing, China.

Huihong Zhai (H)

National Clinical Research Center for Digestive Diseases, Capital Medical University Affiliated Beijing Friendship Hospital, Beijing, China.

Bangmao Wang (B)

Department of Gastroenterology and Hepatology, Tianjin Medical University General Hospital, Tianjin, China.

Yanqing Li (Y)

Department of Gastroenterology, Shandong University Qilu Hospital, Jinan, China.

Junfeng Xu (J)

Senior Department of Gastroenterology, the First Medical Center of Chinese PLA General Hospital, Beijing, China.

Jiheng Wang (J)

Senior Department of Gastroenterology, the First Medical Center of Chinese PLA General Hospital, Beijing, China.
Department of Gastroenterology, the Seventh Medical Center of Chinese PLA General Hospital, Beijing, China.

Yuqi He (Y)

Department of Gastroenterology, the Seventh Medical Center of Chinese PLA General Hospital, Beijing, China.

Yang Wang (Y)

National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College Fuwai Hospital, Beijing, China.

Min Dai (M)

National Cancer Center/National Clinical Research Center for Cancer, Cancer Hospital Chinese Academy of Medical Sciences, Beijing, China.

Jianqiu Sheng (J)

Department of Gastroenterology, the Seventh Medical Center of Chinese PLA General Hospital, Beijing, China.

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