Mandipropamid as a chemical inducer of proximity for in vivo applications.
Journal
Nature chemical biology
ISSN: 1552-4469
Titre abrégé: Nat Chem Biol
Pays: United States
ID NLM: 101231976
Informations de publication
Date de publication:
01 2022
01 2022
Historique:
received:
09
09
2020
accepted:
13
10
2021
pubmed:
23
12
2021
medline:
23
2
2022
entrez:
22
12
2021
Statut:
ppublish
Résumé
Direct control of protein interactions by chemically induced protein proximity holds great potential for both cell and synthetic biology as well as therapeutic applications. Low toxicity, orthogonality and excellent cell permeability are important criteria for chemical inducers of proximity (CIPs), in particular for in vivo applications. Here, we present the use of the agrochemical mandipropamid (Mandi) as a highly efficient CIP in cell culture systems and living organisms. Mandi specifically induces complex formation between a sixfold mutant of the plant hormone receptor pyrabactin resistance 1 (PYR1) and abscisic acid insensitive (ABI). It is orthogonal to other plant hormone-based CIPs and rapamycin-based CIP systems. We demonstrate the applicability of the Mandi system for rapid and efficient protein translocation in mammalian cells and zebrafish embryos, protein network shuttling and manipulation of endogenous proteins.
Identifiants
pubmed: 34934192
doi: 10.1038/s41589-021-00922-3
pii: 10.1038/s41589-021-00922-3
pmc: PMC8709788
doi:
Substances chimiques
Amides
0
Carboxylic Acids
0
Fungicides, Industrial
0
mandipropamid
11GP4ELK0U
Abscisic Acid
72S9A8J5GW
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
64-69Informations de copyright
© 2021. The Author(s).
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