Helical ordering of envelope-associated proteins and glycoproteins in respiratory syncytial virus.
cryo-EM
cryo-ET
glycoprotein
matrix protein
virus structure
Journal
The EMBO journal
ISSN: 1460-2075
Titre abrégé: EMBO J
Pays: England
ID NLM: 8208664
Informations de publication
Date de publication:
01 02 2022
01 02 2022
Historique:
revised:
01
12
2021
received:
15
09
2021
accepted:
03
12
2021
pubmed:
23
12
2021
medline:
17
2
2022
entrez:
22
12
2021
Statut:
ppublish
Résumé
Human respiratory syncytial virus (RSV) causes severe respiratory illness in children and the elderly. Here, using cryogenic electron microscopy and tomography combined with computational image analysis and three-dimensional reconstruction, we show that there is extensive helical ordering of the envelope-associated proteins and glycoproteins of RSV filamentous virions. We calculated a 16 Å resolution sub-tomogram average of the matrix protein (M) layer that forms an endoskeleton below the viral envelope. These data define a helical lattice of M-dimers, showing how M is oriented relative to the viral envelope. Glycoproteins that stud the viral envelope were also found to be helically ordered, a property that was coordinated by the M-layer. Furthermore, envelope glycoproteins clustered in pairs, a feature that may have implications for the conformation of fusion (F) glycoprotein epitopes that are the principal target for vaccine and monoclonal antibody development. We also report the presence, in authentic virus infections, of N-RNA rings packaged within RSV virions. These data provide molecular insight into the organisation of the virion and the mechanism of its assembly.
Identifiants
pubmed: 34935163
doi: 10.15252/embj.2021109728
pmc: PMC8804925
doi:
Substances chimiques
Glycoproteins
0
Viral Matrix Proteins
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e109728Subventions
Organisme : Medical Research Council
ID : MC_U105178939
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 102463/Z/13/Z
Pays : United Kingdom
Organisme : Medical Research Council
ID : MC_U105184322
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
Pays : United Kingdom
Organisme : Medical Research Council
ID : MC_UU_12014/9
Pays : United Kingdom
Organisme : Medical Research Council
ID : MC_UU_12014/7
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/M000451/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : MC-A025-5PL41
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 099786/Z/12/Z
Pays : United Kingdom
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : NIAID NIH HHS
ID : R01 AI113321
Pays : United States
Informations de copyright
© 2021 The Authors Published under the terms of the CC BY 4.0 license.
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