Prediction and Validation of a Protein's Free Energy Surface Using Hydrogen Exchange and (Importantly) Its Denaturant Dependence.


Journal

Journal of chemical theory and computation
ISSN: 1549-9626
Titre abrégé: J Chem Theory Comput
Pays: United States
ID NLM: 101232704

Informations de publication

Date de publication:
11 Jan 2022
Historique:
pubmed: 23 12 2021
medline: 7 4 2022
entrez: 22 12 2021
Statut: ppublish

Résumé

The denaturant dependence of hydrogen-deuterium exchange (HDX) is a powerful measurement to identify the breaking of individual H-bonds and map the free energy surface (FES) of a protein including the very rare states. Molecular dynamics (MD) can identify each partial unfolding event with atomic-level resolution. Hence, their combination provides a great opportunity to test the accuracy of simulations and to verify the interpretation of HDX data. For this comparison, we use

Identifiants

pubmed: 34936354
doi: 10.1021/acs.jctc.1c00960
pmc: PMC8757463
doi:

Substances chimiques

Proteins 0
Hydrogen 7YNJ3PO35Z

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

550-561

Subventions

Organisme : NIGMS NIH HHS
ID : R01 GM055694
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM130122
Pays : United States
Organisme : NIGMS NIH HHS
ID : R29 GM055694
Pays : United States

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Auteurs

Xiangda Peng (X)

Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, Illinois 60637, United States.

Michael Baxa (M)

Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, Illinois 60637, United States.

Nabil Faruk (N)

Graduate Program in Biophysical Sciences, University of Chicago, Chicago, Illinois 60637, United States.

Joseph R Sachleben (JR)

Division of Biological Sciences, University of Chicago, Chicago, Illinois 60637, United States.

Sebastian Pintscher (S)

Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, Illinois 60637, United States.
Department of Molecular Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków 30387, Poland.

Isabelle A Gagnon (IA)

Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, Illinois 60637, United States.

Scott Houliston (S)

Structural Genomics Consortium, University of Toronto, Toronto, Ontario M5G 1L7, Canada.
Princess Margaret Cancer Centre and Department of Medical Biophysics, University of Toronto, Toronto, Ontario M5G 2M9, Canada.

Cheryl H Arrowsmith (CH)

Structural Genomics Consortium, University of Toronto, Toronto, Ontario M5G 1L7, Canada.
Princess Margaret Cancer Centre and Department of Medical Biophysics, University of Toronto, Toronto, Ontario M5G 2M9, Canada.

Karl F Freed (KF)

Department of Chemistry, University of Chicago, Chicago, Illinois 60637, United States.

Gabriel J Rocklin (GJ)

Department of Pharmacology & Center for Synthetic Biology, Northwestern University, Chicago, Illinois 60614, United States.

Tobin R Sosnick (TR)

Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, Illinois 60637, United States.

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