Anti-thymoglobulin induction improves neonatal porcine xenoislet engraftment and survival.


Journal

Xenotransplantation
ISSN: 1399-3089
Titre abrégé: Xenotransplantation
Pays: Denmark
ID NLM: 9438793

Informations de publication

Date de publication:
11 2021
Historique:
revised: 13 07 2021
received: 27 05 2021
accepted: 07 09 2021
entrez: 24 12 2021
pubmed: 25 12 2021
medline: 28 1 2022
Statut: ppublish

Résumé

Porcine islet xenotransplantation is a viable strategy to treat diabetes. Its translation has been limited by the pre-clinical development of a clinically available immunosuppressive regimen. We tested two clinically relevant induction agents in a non-human primate (NHP) islet xenotransplantation model to compare depletional versus nondepletional induction immunosuppression. Neonatal porcine islets were isolated from GKO or hCD46/GKO transgenic piglets and transplanted via portal vein infusion in diabetic rhesus macaques. Induction therapy consisted of either basiliximab (n = 6) or rhesus-specific anti-thymocyte globulin (rhATG, n = 6), combined with a maintenance regimen using B7 costimulation blockade, tacrolimus with a delayed transition to sirolimus, and mycophenolate mofetil. Xenografts were monitored by blood glucose levels and porcine C-peptide measurements. Of the six receiving basiliximab induction, engraftment was achieved in 4 with median graft survival of 14 days. All six receiving rhATG induction engrafted with significantly longer xenograft survival at 40.5 days (P = 0.03). These data suggest that depletional induction provides superior xenograft survival to nondepletional induction, in the setting of a costimulation blockade-based maintenance regimen.

Identifiants

pubmed: 34951057
doi: 10.1111/xen.12713
pmc: PMC8715890
mid: NIHMS1741381
doi:

Substances chimiques

Antilymphocyte Serum 0
Immunosuppressive Agents 0

Types de publication

Journal Article Research Support, N.I.H., Extramural

Langues

eng

Sous-ensembles de citation

IM

Pagination

e12713

Subventions

Organisme : NIAID NIH HHS
ID : U01 AI090956
Pays : United States
Organisme : NIAID NIH HHS
ID : HHSN272201300031C
Pays : United States

Commentaires et corrections

Type : ErratumIn

Informations de copyright

© 2021 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

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Auteurs

Qimeng Gao (Q)

Department of Surgery, Duke University School of Medicine, Durham, North Carolina, 27710, USA.

Robert Davis (R)

Department of Surgery, Duke University School of Medicine, Durham, North Carolina, 27710, USA.

Zachary Fitch (Z)

Department of Surgery, Duke University School of Medicine, Durham, North Carolina, 27710, USA.

Michael Mulvihill (M)

Department of Surgery, Duke University School of Medicine, Durham, North Carolina, 27710, USA.

Brian Ezekian (B)

Department of Surgery, Duke University School of Medicine, Durham, North Carolina, 27710, USA.

Paul Schroder (P)

Department of Surgery, Duke University School of Medicine, Durham, North Carolina, 27710, USA.

Robin Schmitz (R)

Department of Surgery, Duke University School of Medicine, Durham, North Carolina, 27710, USA.

Mingqing Song (M)

Department of Surgery, Duke University School of Medicine, Durham, North Carolina, 27710, USA.

Frank Leopardi (F)

Department of Surgery, Duke University School of Medicine, Durham, North Carolina, 27710, USA.

Marianna Ribeiro (M)

Department of Surgery, Duke University School of Medicine, Durham, North Carolina, 27710, USA.

Allison Miller (A)

Department of Surgery, Duke University School of Medicine, Durham, North Carolina, 27710, USA.

Dimitrios Moris (D)

Department of Surgery, Duke University School of Medicine, Durham, North Carolina, 27710, USA.

Brian Shaw (B)

Department of Surgery, Duke University School of Medicine, Durham, North Carolina, 27710, USA.

Kannan Samy (K)

Department of Surgery, Duke University School of Medicine, Durham, North Carolina, 27710, USA.

Keith Reimann (K)

MassBiologics, University of Massachusetts Medical School, Worcester, Massachusetts, 01655, USA.

Kyha Williams (K)

Department of Surgery, Duke University School of Medicine, Durham, North Carolina, 27710, USA.

Bradley Collins (B)

Department of Surgery, Duke University School of Medicine, Durham, North Carolina, 27710, USA.

Allan D Kirk (AD)

Department of Surgery, Duke University School of Medicine, Durham, North Carolina, 27710, USA.

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Classifications MeSH