The Dynamics of Circulating Heparin-Binding Protein: Implications for Its Use as a Biomarker.
Biomarkers
Heparin-binding protein
Sepsis
Journal
Journal of innate immunity
ISSN: 1662-8128
Titre abrégé: J Innate Immun
Pays: Switzerland
ID NLM: 101469471
Informations de publication
Date de publication:
2022
2022
Historique:
received:
17
09
2021
accepted:
12
11
2021
pubmed:
30
12
2021
medline:
9
9
2022
entrez:
29
12
2021
Statut:
ppublish
Résumé
Heparin-binding protein (HBP) is a promising biomarker for the development and severity of sepsis. To guide its use, it is important to understand the factors that could lead to false-positive or negative results, such as inappropriate release and inadequate clearance of HBP. HBP is presumably released only by neutrophils, and the organs responsible for its elimination are unknown. In this study, we aimed to determine whether non-neutrophil cells can be a source of circulating HBP and which organs are responsible for its removal. We found that in two cohorts of neutropenic patients, 12% and 19% of patients in each cohort, respectively, had detectable plasma HBP levels. In vitro, three leukemia-derived monocytic cell lines and healthy CD14+ monocytes constitutively released detectable levels of HBP. When HBP was injected intravenously in rats, we found that plasma levels of HBP decreased rapidly, with a distribution half-life below 10 min and an elimination half-life of 1-2 h. We measured HBP levels in the liver, spleen, kidneys, lungs, and urine using both ELISA and immunofluorescence quantitation, and found that the majority of HBP was present in the liver, and a small amount was present in the spleen. Immunofluorescence imaging indicated that HBP is associated mainly with hepatocytes in the liver and monocytes/macrophages in the spleen. The impact of hematologic malignancies and liver diseases on plasma HBP levels should be explored further in clinical studies.
Identifiants
pubmed: 34965528
pii: 000521064
doi: 10.1159/000521064
pmc: PMC9485916
doi:
Substances chimiques
AZU1 protein, human
0
Antimicrobial Cationic Peptides
0
Biomarkers
0
Blood Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
447-460Informations de copyright
© 2021 The Author(s). Published by S. Karger AG, Basel.
Références
Eur J Cell Biol. 2014 Aug-Sep;93(8-9):347-54
pubmed: 25172775
PLoS One. 2019 Aug 29;14(8):e0221813
pubmed: 31465432
J Immunol. 1998 Jun 1;160(11):5530-6
pubmed: 9605157
Ann Intensive Care. 2017 Oct 18;7(1):105
pubmed: 29047023
PLoS One. 2016 Nov 28;11(11):e0164489
pubmed: 27893741
Crit Care Med. 2015 Nov;43(11):2378-86
pubmed: 26468696
J Intern Med. 2017 Jun;281(6):562-574
pubmed: 28370601
J Clin Invest. 2008 Oct;118(10):3491-502
pubmed: 18787642
Front Immunol. 2019 Apr 16;10:834
pubmed: 31040854
J Mol Biol. 1990 Oct 5;215(3):403-10
pubmed: 2231712
Eur J Haematol. 2017 Dec;99(6):559-568
pubmed: 28952164
Clin Infect Dis. 2009 Oct 1;49(7):1044-50
pubmed: 19725785
J Cardiothorac Vasc Anesth. 2021 Sep;35(9):2640-2650
pubmed: 33454168
Pflugers Arch. 2007 Jun;454(3):345-59
pubmed: 17256154
BMC Med Genomics. 2008 Sep 15;1:41
pubmed: 18793429
J Exp Med. 2000 Jun 5;191(11):1829-39
pubmed: 10839800
J Exp Med. 1989 Mar 1;169(3):833-45
pubmed: 2538548
Crit Care. 2012 May 21;16(3):R90
pubmed: 22613179
Shock. 2017 Sep;48(3):313-320
pubmed: 28319494
Am J Respir Crit Care Med. 2017 Oct 1;196(7):856-863
pubmed: 28345952
Kidney Int. 1978 Apr;13(4):278-88
pubmed: 651127
Am J Pathol. 2002 Mar;160(3):841-8
pubmed: 11891183
J Biol Chem. 1995 Feb 10;270(6):2841-5
pubmed: 7852358
Fed Proc. 1966 Nov-Dec;25(6):1773-83
pubmed: 5927412
J Leukoc Biol. 1999 Dec;66(6):989-95
pubmed: 10614782
Crit Care. 2015 Jan 28;19:26
pubmed: 25887223
Intensive Care Med Exp. 2020 Sep 10;8(1):51
pubmed: 32910266
J Clin Invest. 1999 Oct;104(7):885-94
pubmed: 10510329
Biochem Biophys Res Commun. 2002 Apr 26;293(1):213-9
pubmed: 12054586
J Clin Oncol. 2000 Aug;18(16):3038-51
pubmed: 10944139
Neurobiol Aging. 1996 Sep-Oct;17(5):753-9
pubmed: 8892348
PLoS One. 2018 May 3;13(5):e0196423
pubmed: 29723248
Biochemistry (Mosc). 2010 Nov;75(11):1361-7
pubmed: 21314603
Haematologica. 2006 Feb;91(2):215-22
pubmed: 16461306
Invest Ophthalmol Vis Sci. 2008 Feb;49(2):726-31
pubmed: 18235021
Intensive Care Med Exp. 2016 Dec;4(1):33
pubmed: 27704481
J Leukoc Biol. 1999 Oct;66(4):634-43
pubmed: 10534120
J Leukoc Biol. 1996 Sep;60(3):415-22
pubmed: 8830799
BMC Pulm Med. 2013 Jul 19;13:47
pubmed: 23870614
Physiol Rev. 2008 Jul;88(3):887-918
pubmed: 18626063
Crit Care Med. 2013 Mar;41(3):820-32
pubmed: 23348516
Shock. 2019 Dec;52(6):e135-e145
pubmed: 30807529
Nucleic Acids Res. 2021 Jan 8;49(D1):D480-D489
pubmed: 33237286
Acta Crystallogr D Biol Crystallogr. 1998 Jul 1;54(Pt 4):598-609
pubmed: 9761855
J Immunol. 1999 Apr 1;162(7):4240-5
pubmed: 10201953
Cell Rep. 2019 Nov 19;29(8):2505-2519.e4
pubmed: 31747616
Blood. 2002 Mar 1;99(5):1785-93
pubmed: 11861296
Inflamm Res. 2012 Apr;61(4):375-9
pubmed: 22207392
Biochem J. 1978 Jul 15;174(1):53-9
pubmed: 29608
Methods Enzymol. 2010;479:223-41
pubmed: 20816169
Vaccines (Basel). 2019 Nov 01;7(4):
pubmed: 31683930