Development of an Automatic Measurement Method for CD8 and PD-1 Positive T Cells Using Image Analysis Software.
Antibodies, Anti-Idiotypic
/ immunology
CD8 Antigens
/ genetics
CD8-Positive T-Lymphocytes
/ metabolism
Colorectal Neoplasms
/ genetics
Humans
Image Processing, Computer-Assisted
Immune Checkpoint Inhibitors
/ therapeutic use
Lymphocytes, Tumor-Infiltrating
/ pathology
Male
Polymorphism, Single Nucleotide
/ genetics
Programmed Cell Death 1 Receptor
/ genetics
Software
Stomach Neoplasms
/ genetics
Tumor microenvironment (TME)
automatic measurement
digital pathology
imaging analysis software
project HOPE
tumor-infiltrating lymphocytes (TILs)
Journal
Anticancer research
ISSN: 1791-7530
Titre abrégé: Anticancer Res
Pays: Greece
ID NLM: 8102988
Informations de publication
Date de publication:
Jan 2022
Jan 2022
Historique:
received:
29
10
2021
revised:
19
11
2021
accepted:
22
11
2021
entrez:
31
12
2021
pubmed:
1
1
2022
medline:
13
1
2022
Statut:
ppublish
Résumé
With the progress in cancer immunotherapy using immune checkpoint blockade (ICB) therapy, histological observations of tumor-infiltrating lymphocyte (TIL) status are needed to evaluate the antitumor effect of ICB using imaging analysis software. Formalin-fixed paraffin-embedded sections obtained from colorectal cancer and gastric cancer patients with more than 500 single nucleotide variants were stained with anti-CD8 and anti-PD-1 antibodies. Based on our own algorithm and imaging analysis software, an automatic TIL measurement method was established and compared to the manual counting methods. In the CD8 An imaging software-based automatic measurement could be a simple and useful tool for evaluating the therapeutic effect of cancer immunotherapies in terms of TIL status.
Sections du résumé
BACKGROUND/AIM
OBJECTIVE
With the progress in cancer immunotherapy using immune checkpoint blockade (ICB) therapy, histological observations of tumor-infiltrating lymphocyte (TIL) status are needed to evaluate the antitumor effect of ICB using imaging analysis software.
MATERIALS AND METHODS
METHODS
Formalin-fixed paraffin-embedded sections obtained from colorectal cancer and gastric cancer patients with more than 500 single nucleotide variants were stained with anti-CD8 and anti-PD-1 antibodies. Based on our own algorithm and imaging analysis software, an automatic TIL measurement method was established and compared to the manual counting methods.
RESULTS
RESULTS
In the CD8
CONCLUSION
CONCLUSIONS
An imaging software-based automatic measurement could be a simple and useful tool for evaluating the therapeutic effect of cancer immunotherapies in terms of TIL status.
Identifiants
pubmed: 34969752
pii: 42/1/419
doi: 10.21873/anticanres.15500
doi:
Substances chimiques
Antibodies, Anti-Idiotypic
0
CD8 Antigens
0
Immune Checkpoint Inhibitors
0
PDCD1 protein, human
0
Programmed Cell Death 1 Receptor
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
419-427Informations de copyright
Copyright © 2022 International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.