Detection of early myocardial cell death in owl monkeys (Aotus nancymai) using complement component C9 immunohistochemistry in formalin-fixed paraffin-embedded heart tissues: A retrospective study.
Aotus spp
cardiomyopathy
catecholamine toxicity
immunohistochemical stain
infarction
myocardium
Journal
Journal of medical primatology
ISSN: 1600-0684
Titre abrégé: J Med Primatol
Pays: Denmark
ID NLM: 0320626
Informations de publication
Date de publication:
04 2022
04 2022
Historique:
received:
02
02
2021
accepted:
20
12
2021
pubmed:
1
1
2022
medline:
10
5
2022
entrez:
31
12
2021
Statut:
ppublish
Résumé
Owl monkeys are commonly used in biomedical research which is affected by the high incidence of cardiomyopathy in this species. Occasionally, owl monkeys with no clinical signs of heart disease are found dead and at necropsy show no, or very mild, cardiomyopathy. A possible explanation for sudden death is acute myocardial infarction; however, early myocardial changes may be difficult to assess by conventional stains and light microscopy. Complement component C9 immunohistochemistry was performed in paraffin-embedded heart tissue samples from owl monkeys who died suddenly, or were euthanized due to sickness, to determine whether these animals suffered from acute myocardial infarcts. C9 deposits were found in the myocardium of 19 out of 20 (95%) animals. The findings in this study suggest owl monkeys suffer from acute myocardial infarcts, and complement component C9 immunohistochemistry may be a useful diagnostic tool.
Sections du résumé
BACKGROUND
Owl monkeys are commonly used in biomedical research which is affected by the high incidence of cardiomyopathy in this species. Occasionally, owl monkeys with no clinical signs of heart disease are found dead and at necropsy show no, or very mild, cardiomyopathy. A possible explanation for sudden death is acute myocardial infarction; however, early myocardial changes may be difficult to assess by conventional stains and light microscopy.
METHODS
Complement component C9 immunohistochemistry was performed in paraffin-embedded heart tissue samples from owl monkeys who died suddenly, or were euthanized due to sickness, to determine whether these animals suffered from acute myocardial infarcts.
RESULTS AND CONCLUSION
C9 deposits were found in the myocardium of 19 out of 20 (95%) animals. The findings in this study suggest owl monkeys suffer from acute myocardial infarcts, and complement component C9 immunohistochemistry may be a useful diagnostic tool.
Identifiants
pubmed: 34971004
doi: 10.1111/jmp.12567
pmc: PMC8897264
mid: NIHMS1767291
doi:
Substances chimiques
Formaldehyde
1HG84L3525
Types de publication
Journal Article
Research Support, N.I.H., Intramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
93-100Subventions
Organisme : Intramural NIH HHS
ID : Z99 AI999999
Pays : United States
Organisme : NIH HHS
Pays : United States
Informations de copyright
Published 2021. This article is a U.S. Government work and is in the public domain in the USA.
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