Tissue Glycome Mapping: Lectin Microarray-Based Differential Glycomic Analysis of Formalin-Fixed Paraffin-Embedded Tissue Sections.

Formaldehyde Glycomics / methods Lectins / metabolism Microarray Analysis Paraffin Embedding Tissue Fixation
Cell type-selective analysis Formalin-fixed paraffin-embedded tissue section Histochemistry Laser microdissection Lectin microarray Tissue glycome mapping

Journal

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969

Informations de publication

Date de publication:
2022
Historique:
entrez: 1 1 2022
pubmed: 2 1 2022
medline: 1 4 2022
Statut: ppublish

Résumé

Lectin microarray (LMA) is a high-sensitive glycan analysis technology used to obtain global glycomic profiles of both N- and O-glycans attached not only to purified glycoproteins but also to crude glycoprotein samples. Through additional use of laser microdissection (LMD) for tissue collection, we developed an LMA-based glycomic profiling technique for a specific type of cells in a tiny area of formalin-fixed paraffin-embedded (FFPE) tissue sections. This LMD-LMA method makes it possible to obtain reproducible tissue glycomic profiles that can be compared with each other, using a unified protocol for all procedures, including FFPE tissue preparation, tissue staining, protein extraction and labeling, and LMA analysis. Here, we describe the standardized LMD-LMA procedure for a "tissue glycome mapping" approach, which facilitates an in-depth understanding of region- and tissue-specific protein glycosylation. We also describe potential applications of the spatial tissue glycomic profiles, including histochemical analysis for evaluating distribution of lectin ligands and a fluorescence LMD-LMA method for cell type-selective glycomic profiling using a cell type-specific probe, composed of a lectin and an antibody. The protocols presented here will accelerate the effective utilization of FFPE tissue specimens by providing tissue glycome maps for the discovery of the biological roles and disease-related alterations of protein glycosylation.

Identifiants

DOI: 10.1007/978-1-0716-2148-6_10 PMID: 34972936
pubmed: 34972936
doi: 10.1007/978-1-0716-2148-6_10
doi:

Substances chimiques

Lectins 0
Formaldehyde 1HG84L3525

Types de publication

Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Langues

eng

Sous-ensembles de citation

IM

Pagination

161-180

Informations de copyright

© 2022. Springer Science+Business Media, LLC, part of Springer Nature.

Références

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Auteurs

Chiaki Nagai-Okatani (C)

Glycoscience and Glycotechnology Research Group, Biotechnology Research Institute for Drug Discovery, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan. chiaki-okatani@aist.go.jp.

Xia Zou (X)

Glycoscience and Glycotechnology Research Group, Biotechnology Research Institute for Drug Discovery, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan.
Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University, Shanghai, China.

Atsushi Matsuda (A)

Glycoscience and Glycotechnology Research Group, Biotechnology Research Institute for Drug Discovery, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan.
Department of Biochemistry, School of Medicine, Keio University, Tokyo, Japan.

Yoko Itakura (Y)

Department of Geriatric Medicine (Vascular Medicine), Tokyo Metropolitan Institute of Gerontology, Tokyo, Japan.

Masashi Toyoda (M)

Department of Geriatric Medicine (Vascular Medicine), Tokyo Metropolitan Institute of Gerontology, Tokyo, Japan.

Yan Zhang (Y)

Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University, Shanghai, China.

Atsushi Kuno (A)

Glycoscience and Glycotechnology Research Group, Biotechnology Research Institute for Drug Discovery, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan. atsu-kuno@aist.go.jp.

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Classifications MeSH

questionsmedicales.fr › Composés chimiques organiques › Aldéhydes › Formaldéhyde › Tissue Glycome Mapping: Lectin...
questionsmedicales.fr › Disciplines des sciences naturelles › Chimie › Biochimie › Glycomique › Tissue Glycome Mapping: Lectin...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Lectines › Tissue Glycome Mapping: Lectin...
questionsmedicales.fr › Techniques d'investigation › Procédures d'analyse sur micropuce › Analyse sur microréseau › Tissue Glycome Mapping: Lectin...
questionsmedicales.fr › Techniques d'investigation › Techniques de laboratoire clinique › Techniques histologiques › Techniques de préparation histocytologique › Inclusion de tissu › Inclusion en paraffine › Tissue Glycome Mapping: Lectin...
questionsmedicales.fr › Techniques d'investigation › Techniques de laboratoire clinique › Techniques histologiques › Techniques de préparation histocytologique › Fixation tissulaire › Tissue Glycome Mapping: Lectin...