Induction and Detection of Mitophagy.
Autophagy
Confocal microscopy
Flow cytometry
Mitochondria
Mitophagy
Respiration
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2022
2022
Historique:
entrez:
1
1
2022
pubmed:
2
1
2022
medline:
1
4
2022
Statut:
ppublish
Résumé
Mitophagy, a process of selective elimination of mitochondria by autophagy, is a mechanism of mitochondrial quality control that maintains mitochondrial network functionality. The elimination of damaged mitochondria through autophagy requires two steps: induction of general autophagy and priming of damaged mitochondria for selective autophagic recognition. Mitophagy impairment is linked to various pathologies; thus, removal of malfunctioning or even harmful mitochondria is vital to cellular physiology. Here, we describe methods that can be applied to the investigation of mitophagy.
Identifiants
pubmed: 34972995
doi: 10.1007/978-1-0716-2071-7_14
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
227-239Informations de copyright
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
Références
Nguyen TN, Padman BS, Lazarou M (2016) Deciphering the molecular signals of PINK1/Parkin mitophagy. Trends Cell Biol 26:733–744. https://doi.org/10.1016/j.tcb.2016.05.008
doi: 10.1016/j.tcb.2016.05.008
pubmed: 27291334
Vara-Perez M, Felipe-Abrio B, Agostinis P (2019) Mitophagy in cancer: a tale of adaptation. Cells 8:493. https://doi.org/10.3390/cells8050493
doi: 10.3390/cells8050493
pmcid: 6562743
Ibáñez P, Lesage S, Lohmann E et al (2006) Mutational analysis of the PINK1 gene in early-onset parkinsonism in Europe and North Africa. Brain 129:686–694. https://doi.org/10.1093/brain/awl005
doi: 10.1093/brain/awl005
pubmed: 16401616
Kerr JS, Adriaanse BA, Greig NH et al (2017) Mitophagy and Alzheimer’s disease: cellular and molecular mechanisms. Trends Neurosci 40:151–166. https://doi.org/10.1016/j.tins.2017.01.002
doi: 10.1016/j.tins.2017.01.002
pubmed: 28190529
pmcid: 5341618
Li X, Huang L, Lan J et al (2021) Molecular mechanisms of mitophagy and its roles in neurodegenerative diseases. Pharmacol Res 163:105240. https://doi.org/10.1016/j.phrs.2020.105240
doi: 10.1016/j.phrs.2020.105240
pubmed: 33053441
Luo H, Zhang R, Krigman J et al (2020) A healthy heart and a healthy brain: looking at mitophagy. Front Cell Dev Biol 8:294. https://doi.org/10.3389/fcell.2020.00294
doi: 10.3389/fcell.2020.00294
pubmed: 32435642
pmcid: 7218083
Bernardini JP, Lazarou M, Dewson G (2017) Parkin and mitophagy in cancer. Oncogene 36:1315–1327. https://doi.org/10.1038/onc.2016.302
doi: 10.1038/onc.2016.302
pubmed: 27593930
Wu L, Zhang D, Zhou L et al (2019) FUN14 domain-containing 1 promotes breast cancer proliferation and migration by activating calcium-NFATC1-BMI1 axis. EBioMedicine 41:384–394. https://doi.org/10.1016/j.ebiom.2019.02.032
doi: 10.1016/j.ebiom.2019.02.032
pubmed: 30803933
pmcid: 6442990
Hui L, Wu H, Wang T-W et al (2019) Hydrogen peroxide-induced mitophagy contributes to laryngeal cancer cells survival via the upregulation of FUNDC1. Clin Transl Oncol 21:596–606. https://doi.org/10.1007/s12094-018-1958-5
doi: 10.1007/s12094-018-1958-5
pubmed: 30284230
Hou H, Er P, Cheng J et al (2017) High expression of FUNDC1 predicts poor prognostic outcomes and is a promising target to improve chemoradiotherapy effects in patients with cervical cancer. Cancer Med 6:1871–1881. https://doi.org/10.1002/cam4.1112
doi: 10.1002/cam4.1112
pubmed: 28719148
pmcid: 5548885
Lemasters JJ (2005) Selective mitochondrial autophagy, or mitophagy, as a targeted defense against oxidative stress, mitochondrial dysfunction, and aging. Rejuvenation Res 8:3–5. https://doi.org/10.1089/rej.2005.8.3
doi: 10.1089/rej.2005.8.3
pubmed: 15798367
Sowter HM, Ratcliffe PJ, Watson P et al (2001) HIF-1-dependent regulation of hypoxic induction of the cell death factors BNIP3 and NIX in human tumors. Cancer Res 61:6669–6673
pubmed: 11559532
Mooney RA (1988) [18] Use of digitonin-permeabilized adipocytes for cAMP studies, pp 193–202
Mauro-Lizcano M, Esteban-Martínez L, Seco E et al (2015) New method to assess mitophagy flux by flow cytometry. Autophagy 11:833–843. https://doi.org/10.1080/15548627.2015.1034403
doi: 10.1080/15548627.2015.1034403
pubmed: 25945953
pmcid: 4509449
Jin SM, Lazarou M, Wang C, Kane LA, Narendra DP, Youle RJ. Mitochondrial membrane potential regulates PINK1 import and proteolytic destabilization by PARL. J Cell Biol. 2010 Nov 29;191(5):933-42. doi:10.1083/jcb.201008084. PMID: 21115803; PMCID: PMC2995166.