Systems Approaches in the Common Metabolomics in Acute Lymphoblastic Leukemia and Rhabdomyosarcoma Cells: A Computational Approach.


Journal

Advances in experimental medicine and biology
ISSN: 0065-2598
Titre abrégé: Adv Exp Med Biol
Pays: United States
ID NLM: 0121103

Informations de publication

Date de publication:
2021
Historique:
entrez: 1 1 2022
pubmed: 2 1 2022
medline: 5 1 2022
Statut: ppublish

Résumé

Acute lymphoblastic leukemia is the most common childhood malignancy. Rhabdomyosarcoma, on the other hand, is a rare type of malignancy which belongs to the primitive neuroectodermal family of tumors. The aim of the present study was to use computational methods in order to examine the similarities and differences of the two different tumors using two cell lines as a model, the T-cell acute lymphoblastic leukemia CCRF-CEM and rhabdomyosarcoma TE-671, and, in particular, similarities of the metabolic pathways utilized by two different cell types in vitro. Both cell lines were studied using microarray technology. Differential expression profile has revealed genes with similar expression, suggesting that there are common mechanisms between the two cell types, where some of these mechanisms are preserved from their ancestor embryonic cells. Expression of identified species was modeled using known functions, in order to find common patterns in metabolism-related mechanisms. Species expression manifested very interesting dynamics, and we were able to model the system with elliptical/helical functions. We discuss the results of our analysis in the context of the commonly occurring genes between the two cell lines and the respective participating pathways as far as extracellular signaling and cell cycle regulation/proliferation are concerned. In the present study, we have developed a methodology, which was able to unravel some of the underlying dynamics of the metabolism-related species of two different cell types. Such approaches could prove useful in understanding the mechanisms of tumor ontogenesis, progression, and proliferation.

Identifiants

pubmed: 34973010
doi: 10.1007/978-3-030-78775-2_8
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

55-66

Informations de copyright

© 2021. The Author(s), under exclusive license to Springer Nature Switzerland AG.

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Auteurs

Tselios C (T)

National and Kapodistrian University of Athens, Laboratory for the Research of Musculoskeletal Disorders, Athens, Greece.

Apostolos Zaravinos (A)

European University Cyprus, Department of Life Sciences, Nicosia CY, Cyprus.

Athanasios N Tsartsalis (AN)

Naval Hospital of Athens, Department of Endocrinology Diabetes and Metabolism, Athens, Greece.

Anna Tagka (A)

First Department of Dermatology and Venereology, "Andreas Syggros" Hospital National and Kapodistrian University of Athens, Athens, Greece.

Athanasios Kotoulas (A)

National Technical University of Athens, School of Electrical and Computer Engineering, Biomedical Engineering Laboratory, Athens, Greece.

Styliani A Geronikolou (SA)

Biomedical Research Foundation of Academy of Athens, Clinical, Translational, Experimental Surgery Research Centerment of Pediatrics, Choremeio Research Laboratory, Athens, Greece.

Maria Braoudaki (M)

Department of Life and Environmental Sciences, School of Life and Health Sciences, University of Hertfordshire, Hatfield, England.

George I Lambrou (GI)

National and Kapodistrian University of Athens, First Department of Pediatrics, Choremeio Research Laboratory, Athens, Greece. glamprou@med.uoa.gr.

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